小肠脂酸联合蛋白基因与2型糖尿病患者体脂含量及散布的....docVIP

小肠脂酸结合蛋白基因与2型糖尿病患者体脂含量及分布的... 小肠脂酸结合蛋白基因与2型糖尿病患者体脂含量及分布的相关性 2010-04-24 项坤三　郑泰山　贾伟平 【摘要】　目的　观察小肠脂酸结合蛋白基因(FABP2)密码子54变异与体脂含量及分布的关系。方法　165例中国人，糖耐量正常(NGT)者86人，2型糖尿病患者(DM)79人。用PCR/Hha I酶解检测FABP2密码子54变异的等位基因——苏氨酸54(Thr54)及丙氨酸54(Ala54)。比较NGT及DM组间及组内体脂含量亚组(BMI)及体脂分布亚组(核磁共振局部体脂测定)间的FABP2密码子54频率分布。结果　DM股部皮下脂肪(FA)亚组间FABP2密码子54变异基因型频率分布有显著差异(χ2=11.46,P=0.003)。表现为FA＜75cm2者Thr54(+)　基因型频率显著增高　(χ2=10.11,P=0.001；比数比4.62)，及Thr54等位基因型频率显著增高(χ2=5.379,P=0.020)。FA相关因素的多元逐步回归分析中见到除性别(P=0.0001)、BMI(P=0.0001)及血总胆固醇(P=0.004)外，FABP2亦　是独立相关因素　(P=0.003)。FABP2相　关因　素的Logistic回归分析中，仅见FA与之相关(P=0.029)。结论　FABP2与体脂分布相关。因为DM不仅有腹内脂肪增多，且有股部脂肪减少；DM股部脂肪含量减少与FABP2 Thr54(+)基因型相关，提示FABP2可能参与糖尿病的病理生理过程。 【关键词】　小肠脂酸结合蛋白　　基因　　密码子变异　　局部体脂含量　　2型糖尿病 The relation of intestinal fatty acid binding protien gene Ala54Thr variation to general and regional adipose depots in type 2 diabetes mellitus Xiang Kunsan,Zheng Taisan,Jia Weiping.Department of Endocrinology and Metabolism,Diabetes Research Laboratory,Medical Genetics Research Laboratory,Shanghai Sixth People Hospital,Shanghai,200233 【Abstract】　Objective　To investigate the association between the codon 54 variation of intestinal fatty acid binding protein (FABP2) gene and the amount and the distribution of adipose depots. Methods　In 165 Chinese subjects 〔86 with normal glucose tolerance (NGT), and 79 with type 2 diabetes (DM)〕. The alleles of the codon 54 variation of FABP2 gene-allele threonine 54 (Thr54) and allele alanine54 (Ala54) were detected by using PCR/Hha I digestion. The frequencies of the FABP2-codon 54 variation were compared between NGT and DM groups, and also between body fat subgroups (expressed as BMI) and body fat distribution subgroups (expressed as regional adipose tissue area measured by magnetic resonance imaging) within NGT and DM groups. Results　The genotype frequencies of FABP2-codon 54 variation were significantly different　　between　　femoral　　subcutaneous　　adipose　　tissue　　(FA)　　subgroups　　in　　DM　　subjects (χ2=11.46,P=0.003). DM subjec