NEB酶切位点保护碱基.pdfVIP

PCR 设计引物时酶切位点的保护 切割率% 酶 寡核苷酸序列 2 hr 20 hr Acc I GGTCGACC 0 0 CGGTCGACCG 0 0 CCGGTCGACCGG 0 0 Afl III CACATGTG 0 0 CCACATGTGG 90 90 CCCACATGTGGG 90 90 Asc I GGCGCGCC 90 90 AGGCGCGCCT 90 90 TTGGCGCGCCAA 90 90 Ava I CCCCGGGG 50 90 CCCCCGGGGG 90 90 TCCCCCGGGGGA 90 90 BamH I CGGATCCG 10 25 CGGGATCCCG 90 90 CGCGGATCCGCG 90 90 Bgl II CAGATCTG 0 0 GAAGATCTTC 75 90 GGAAGATCTTCC 25 90 BssH II GGCGCGCC 0 0 AGGCGCGCCT 0 0 TTGGCGCGCCAA 50 90 BstE II