《微生物学与免疫学》普通微生物学实验.pptVIP

Spore suspension of Bacillus subilis * * I=lg2 D=1 W=(SH +UH) – (SL + UL) V=(UH +UL) – (SH + SL) θ= D×antilog(IV/W) * * * * * * * * fungi medium fluid thioglycollate medium 1 2 3 4 5 6 7 S.aureus Sample Sample 1ml 1ml 1ml 1ml 1ml ck ck ck Procedures —test for sterility of drugs * * 0.9% natural saline injection solution No Media Sample Strain Culture (d) Bacteria Fungi 1 fluid thioglycollate medium 1ml S.aureus 1 2 fluid thioglycollate medium 1ml 0.9%N.S 14 3 fluid thioglycollate medium 1ml 0.9%N.S 14 4 luid thioglycollate medium --- 14 5 fungi medium 1ml 0.9%N.S 14 6 fungi medium 1ml 0.9%N.S 14 7 fungi medium --- 14 “+”growth，“-”no growth * * 10-2 10-5 10-4 10-3 1ml 1ml 1ml 1ml video * * Count clonies:30-300 * * EMB平板典型大肠杆 菌菌落特征： 中心黑色或紫红色，有 或无绿色金属光泽 大肠杆菌测定——EMB选择性分离鉴别 * * * 1. Drop a blob of methylene blue 2.Take some yeast 3.Cover the suspension 4.Examine the slide * * * * Saccharomyces cerevisae * * Agar surface 气生菌丝 基内菌丝 * * 1.Place a drop of methylene blue 2.Press a cover slip on the colony surface 3.Cover the suspension 4.Examine the slide * * 放线菌2809 * * 青霉菌 * * Results and Discussion antinomycetes molds * * 2. Describe the characteristics of Colonies of Actinomycetes 2809, Yeast(Sacchariomyces) and Molds (Penicillum) Results and Discussion 2809 Sacchariomyces Penicillum Size Shape Surface Edge Color Dissolved pigment * * 3. Lawn of Actinomycetes(1~5) Antinomycetes No.1 No. 2 No. 3 No. 4 No. 5 Size Shape Surface Shape and color of aerial mycelia Color of spore mycelia Color of substrate mycelia Dissolved pigment * * 4. Describe the characteristics of Molds(4 Penicillum, Aspergillus, Mucor, Rhizopus) Fungi Penicillum Aspergillus Mucor Rhizopus Sacchariomyces Size Shape Surface Shape and color of aerial mycelia Color of spore mycelia Color of substrate mycelia Dissolved pigment * * * * * * * * 1. Draw a representa