蛋白质入核转运的机制及研究进展.pdf

ISSN 1007-7626 200610 CN 11-3870PQ Chinese Journal of Biochemistry and olecular Biology 22(10):780~ 786 #综述# * 周 鸣, 李小玲, 李桂源 ( 中南大学湘雅医学院肿瘤研究所, 湖南,长沙 410078) 细胞核膜是由外膜和内膜组成的磷脂双 子层结构, 同时镶嵌 一些核孔复合体(NPC). 核孔 复合体是胞浆和胞核之间主动和被动转运的生理屏障.核内功能蛋白在胞浆内合成后通过核孔复 合体进入胞核, 这 个过程除了需要NPC 上核孔蛋白胞浆内核转运受体和RanGTP 等蛋白的参与 外,货物蛋白本身的结构特征在其入核转运过程中亦发挥重要作用.本文着重就蛋白入核转运的机 制及近年来取得的相关进展进行综述. 蛋白质;核孔复合体;核输入 Q6 Molecular Mechanism and Progress of Protein Transport from Cytoplasm to Nucleus * ZHOU ing,LIXiao-Ling,LI Gu-iYuan (Cancer Research I nstitute , Central South University X iang-Ya School of M edicine , Changsha 410078, China) Abstract The nuclear envelope is composed of two lipid bilayers, outer membrane and inner membrane. Nuclear pore complex (NPC) areformed at siteswhere the inner and outer membranes of the nuclear envelope arejoined and it is a physical barrier of any passive and active transport between the cytoplasm and nucleus. Proteinsfunctioned inthenucleus(usedtobe called as Cargoes) are synthesized inthecytoplasmandtransport to the nucleus across nuclear pore complex in an energy-dependent way. This process is mainly regulated by nucleoporins in NPC, importins in cytoplasm and RanGTP. In addition, the structural character of cargo proteins plays essential roles in the transport to nucleus. This review mainly focuses on the molecular mechanism of proteintransportation to the nucleus andrecent progresses. Key words protein; nuclear pore complex(NPC) ;nuclear import ,, . (nuclear pore