HPLC法测定甲麻芩苷那敏片中三组分含量.PDF

2007 219 723 HPLC 白旭东, 赵晓玲 ( , 110023) 18 : 建立反相离子对H PLC 法测定甲麻芩苷那敏片中三组分的含量采用 Diamonsil C 色谱柱, 以 [02%辛 磺酸钠溶液- 三乙胺 ( 100 02, 用磷酸调节pH 值为43) ] - 甲醇 ( 50 50) 和 (40 60) 为流动相, 分别测定盐酸甲麻黄碱黄芩苷及马来酸氯苯那敏, 检测波长为215nm, 流速为 10 mL 1 1 min 盐酸甲麻黄碱黄芩苷及马来酸氯苯那敏的线性范围分别为 16~ 125 g mL (r 09999) 11 ~ 85 g mL1 (r 1000) 及26~ 102 g mL1 ( r 09991) , 平均回收率分别为997% ( RSD 11%) 994% ( RSD 11%) 及 1002% ( RSD 16%) 本方法简单迅速准确, 可用于甲麻芩 苷那敏片中三组分的含量测定 : 甲麻芩苷那敏片; 盐酸甲麻黄碱; 黄芩苷; 马来酸氯苯那敏; 反相离子对高效液相色谱法 : R9272 : A :( 2007) Determinate of Three Composition of Methylephedrine Hydrochloride, Baicalin and ChlorphenamineMaleate Tablets by HPLC Bai Xudong and Zhao Xiaoling ( Liaoning Province lnstitute for Drug Control, Shenyang 110023) ABSTRACT A RP - Ion pair HPLC method for assay of three composition of Methylephedrine Hydrochloride, Baicalin and Chlorphenamine Maleate T ablets was established T he method was carried out on the diamonsil C18 Column, [ 02% sodium Octanesulfonate- triethylamine ( 100 02, adjust pH value to 43 with phosphoric acid) ] - methanol ( 50 50) and (40 60) was used as the mobile phase, to determine Methylephedrine HydrochlorideBaicalin and chlorphenamine Maleate, respectively The 1 detection wavelength was 215 nm and flow rate was 10 mL min The linear range for Methylephedrine HydrochlorideBaicalin and chlorphenamine Maleate were 16~ 125 g mL1 (r 09999) 11~ 85 g mL1 (r 1000) and 26~ 102 g mL1 ( r 09991) , respectivelyThe average recoveries rates of method were 997% ( RSD 11%) 994% ( RSD 11%) and 1002% ( RSD 16%) respectively The method is simple, quick and accurate, it can be used for quality control of three composition in the drug