响应面试验优化微波辅助固定化胰蛋白酶工艺.pdf

※生物工程 食品科学 2017, Vol.38, No.06 117 响应面试验优化微波辅助固定化胰蛋白酶工艺 刘伟华，李 荣，姜子涛* （天津商业大学生物技术与食品科学学院，天津市食品生物技术重点实验室，天津 300134 ） 摘  要：在微波辅助的条件下，以环氧树脂为载体固定化胰蛋白酶。通过单因素试验和响应面分析法确定了微波辅 / 助固定化胰蛋白酶的最佳条件：微波温度30 ℃、给酶量41 mg g、pH 6.2 、微波时间10 min。在此条件下制备的固定 / 化胰蛋白酶活力为322.56 U g树脂，高于传统振荡方法制备的固定化酶活力，将原来20 h 的固定化时间缩短到几分 钟，并且固定化酶具有良好的稳定性，由此可见微波辅助固定化酶是一种高效的固定化方法。 关键词：固定化酶；环氧树脂；微波；响应面；高效 Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology * LIU Weihua, LI Rong, JIANG Zitao (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) Abstract: Trypsin was immobilized onto epoxy resin under microwave irradiation. Using one-factor-at-a-time method and response surface methodology (RSM), the optimal conditions of immobilization were obtained as follows: trypsin / dosage, 41 mg g; microwave irradiation time, 10 min; pH, 6.2; and temperature, 30 ℃. Under these conditions, the activity / of immobilized trypsin was 322.56 U g resin, which was higher than that of traditional immobilized trypsin and the immobilization time was shortened from 20 h to a few minutes. Furthermore, immobilized trypsin had a good stability. Thus, microwave-assisted immobilization provides an efficient way of immobilizing trypsin. Key words: immobilized trypsin; epoxy resin; microwave; response surface methodology; high efficiency