茄科植物叶绿体基因组插入、缺失与核苷酸替代的发生方式.PDFVIP

HEREDITAS (Beijing) 2008 11 , 30(11): 1506―1512 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.01506 唐萍, 王强, 陈建群 , 210093 : 核苷酸替代和indels(插入、缺失统称)发生是进化的重要动力。以茄科植物为研究对象, 探讨茄属中番茄 和马铃薯、烟草属中绒毛状烟草和普通烟草分化时叶绿体基因组indels 和核苷酸替代的发生方式, 以及这两种 突变对基因组造成的影响。结果显示: indels 和核苷酸替代的发生都不是随意的。indels 发生在A+T 丰富的区 域, 1 bp indels 占据总数的 30% 以上, 大部分 indels 都为低于 10 bp 的较短片段。核苷酸替代表现出 Ts( 转 换)/Tv(颠换)偏差, 但T →G, A →C 颠换频率却明显增加。Ts/Tv 比值出现种属特异性, 番茄和马铃薯比较时替代 的Ts/Tv 比值低于绒毛状烟草和普通烟草比较时Ts/Tv 比值。不同物种替代的(A+T)/(G+C) 比值有一定差异, 从 而影响基因组的(G+C)%, 此比值的差异与形成物种的生长习性有一定的关系。 : 茄科植物; 叶绿体; 插入; 缺失; 核苷酸替代 The patterns and influences of insertions, deletionsand nucleotide substitutions in Solanaceae chloroplast genome TANG Ping, WANG Qiang, CHEN Jian-Qun Biological Department, College of Life Science, Nanjing University, Nanjing 210093, China Abstract: Nucleotide substitution and indels (insertions and deletions) events are the major evolutionary driving forces. Comparisons of the indels and nucleotide substitution patterns were made in the chloroplast genomes between Solanum lycopersicum L. and Solanum bulbocastanum L., Nicotiana tomentosiformis L. and Nicotiana tabacum L. in Solanaceae. The influence of mutation on genome composition was analyzed. The indels and substitutions were not randomly distrib- uted throughout the chloroplast genomes. The indels were in AT-rich regions. One base pair indels accounted for above 30% of the total indels. Most of the indels were short of 10 bp. The nucleotide substitutions showed Ts/Tv bias, but transversion frequency of T G and A C was increased significant