接合、转化、转染、转导的差别(国外英文资料).docVIP

接合、转化、转染、转导的区别(国外英文资料) Differences in conjugation, transformation, transfection, transduction What are the differences between transformation, transduction, and transfection? -------------------------------------------------------------------------------- Transformation (transformation) refers to the introduction of plasmids or other exogenous DNA into a host cell in a receptive state and enables it to acquire a new phenotype. Transfection (transfection) method using similar receptor cells transformed with a plasmid DNA, namely host bacteria after CaCl2, electroporation treatment into competent bacteria, the recombinant phage DNA directly into the receptor cells, into phage DNA competent bacteria can also copy and reproduce, this way is called transfection. Transfection is a special form of transformation. Transduction Transduction is the phenomenon of recombination caused by the transfer of a host of DNA to another host cell. If the donor DNA does not recombine with the receptor DNA, this transduction process is called abortive transduction 1, Conjugation Chinese translation as combination Conjugation is the mechanism by which genetic material is transferred between two bacterial cells by plasmids. A cell containing a conjugative plasmid (F+, fertility+) forms a mating pair with a cell that does not contain a conjugative-plasmid (F-) by means of an F-pilus on the surface of the cell.The pilus contracts, pulling the two cells into contact. And the conjugative plasmids (typified by the F plasmid of E.coli is from the plasmid-containing) transferred cell, the donor, to the recipient (in some cases, pieces of chromosomal DNA is also transfered to the recipient The TRA). Genes, carried on the F plasmid, contain all the information for the conjugative process. Instant, notes, in, Microbiology p125 Explanation: Conjugation occurs in prokaryotes, and the key to this definition is the direct contact between cells such as The, pilus, contracts, pulling, the, two, into, cont