氧化损伤对3T3-L1脂肪细胞内质网应激相关分子及脂联素、MCP-1、PAI-1表达的影响.pdfVIP

increase (P0.01)in PAI-1 mRNA.③a time-course manner in adiponectin ,MCP-1 and PAI-1 mRNA expressions after 10-min exposure to 500µM t-BHP. the adiponectin mRNA level was down-regulated to 53% (P0.01)at 8h and 14% (P0.01)at 24h after exposure to t-BHP for 10min, Significant increase in MCP-1(683%) (P0.01)and PAI-1(539%)(P0.01) mRNA levels after 4h treatment with t-BHP for 10min. CONCLUSION: Oxidative damage actived endoplasmic reticulum stress -related protein,decreased gene expression of adiponectin and increased MCP-1, PAI-1 mRNA expression,all of which could result in adipocytes dysfunction. KEYWORDS: t-BHP; 3T3-L1adipocyte; Adiponectin; MCP-1; PAI-1; endoplasmic reticulum stress. 7 缩略词表（Abbreviations and acronyms ） ATF-6, Activating transcription factor-6, 转录激活因子6 t-BHP, Tert-butylhydroperoxide, 第三丁基过氧化氢 BIP, Heavy-chain binding protein, 内质网分子伴侣重链结合蛋白 BSA, Bovine serum albumin, 牛血清白蛋白 DEX, dexamethasone, 地塞米松 DMEM, Dulbecco’s modified Eagel’s medium, DMEM 培养基 DMSO, dimethyl sulfoxide, 二甲基亚砜 ERS, Endoplasmic reticulum stress, 内质网应激反应 FBS, fetal bovine serum, 胎牛血清 IBMX, 3-isobutyl-1-methylxanthine, 异丁基- 甲基-黄嘌呤 IRE1, Inositol requiring enzyme-1, 肌醇需求蛋白-1 JNK, c-Jun N-terminal kinase , c-Jun 氨基末端激酶 MCP-1, monocyte chemotactic protein 1, 单核细胞趋化蛋白-1 MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, 噻唑蓝 PAI-1, plasminogen activator inhibitor type-1, 1 型纤溶酶原激活物抑制物 PBS, phosphate buffered saline, 磷酸盐缓冲液 PERK, PKR-likeendoplasmic reticulum kinase, 类PKR 的内质网激酶 ROS, reactive oxygen species, 活性氧簇 TRAF2, Tumor necrosis factor receptor associated factor2, TNF 受体相关因子2 XPB-1, X-box Binging protein, X 盒结合蛋白-1