清胰汤对重症急性胰腺炎大鼠结肠肌间及黏膜下神经节VIP神经元的影响.pdfVIP

组为 5.9±0.6 。小肠推进比在假手术组为 53.32±4.67% ，模型组为 17.21±3.98%，清胰汤组为 29.41±1.29%。模型组的小肠推进比较假手术组 减退(P0.05) 。结论: 通过灌服清胰汤来改善SAP 早期胃肠动力紊乱，可能 与肠神经系统VIP 阳性神经元重塑有关。 关键词：重症急性胰腺炎；清胰汤；肠神经系统；血管活性肠肽 II Effects of Qing Yi Decoction on vasoactive intestinal peptide-immunoreactive neurons in rat colonic myenteric ganglia and submucosal ganglia after severe acute pancreatitis ABSTRACT Objective：To observe changes of vasoactive intestinal peptide -immunoreactive (VIP-IR) neurons in colonic enteric nervous system (ENS) after treatment with traditional Chinese medition Qing Yi Decoction in rat severe acute pancreatitis. Methods: Thirty female Sprague-Dawley rats (200g～230g) were randomly divided into three groups: a sham operated group (n=10), a SAP group (n=10), a SAP group treated with Qing Yi Decoction (QYD group) (n=10). SAP model was induced by retrograde cholangiopancreatic duct injection of 5% sodium taurocholate. Rats in Sham operated group and SAP group were introgastrically given saline 1h, 2h and 3h after operation. Rats in QYD group were introgastrically given Qing Yi Decoction 1h, 2h and 3h after SAP induced. Intestinal transit and Pathological damage of pancreas and colonic III mucosa was studied. Double-immunolabeling for Hu-IR neurons and VIP-IR neurons was employed for the whole-mont preperation of colonic submucosal ganglia and colonic myenteric ganglia in ENS. Results: In sham operated rats, Hu-IR neurons were 25.13±7.90 per myenteric ganglion and 12.48±5.01 per submucosal ganglion. The percentage of VIP-IR neurons per myenteric ganglion was lower than per submucosal ganglion