固定化酶法制备单葡萄糖醛酸甘草次酸.docVIP

固定化酶法制备单葡萄糖醛酸甘草次酸 摘 要 甘草酸（glycyrrhizic acid, GL）是甘草的主要有效成分，具有抗炎症，抗病毒，抗肿瘤，抗过敏等作用，而且具有较好的甜味性能。甘草酸在β-葡萄糖醛酸苷酶β-Glucuronidase）作用下能特异的水解glycyrrhetic acid 3-O-mono-?-D-glucuronide, GAMG），作为GL的衍生物，其在抗炎和抗过敏活性上等同或超过GL，而且具有显著的抗癌作用，同时GAMG是一种新型的天然甜味剂，甜度约是GL的5倍。 固定化技术是指将具有化学催化活性的生物催化剂（酶或细胞）进行固定，即定位于一定的空间范围内，实现催化反应。固定化技术可以克服自由酶的不足，使酶可以回收及重复使用，从而成为生物技术中最为活跃的研究领域之一。 利用固定化β-葡萄糖醛酸苷酶β-葡萄糖醛酸苷酶β-葡萄糖醛酸苷酶β-葡萄糖醛酸苷酶 固定化 甘草酸 单葡萄糖醛酸甘草次酸  Preparation of glycyrrhetic acid mono glucuronide by immobilized β-glucuronidase ABSTRACT Glycyrrihizic acid(GL) is a major ingredient of licorice, and it is known for its physiological activities, such as anti-inflammation, antitumor, anti-allergy and antivirus effects. Glycyrrihizic acid was hydrolysed by β-glucuronidase, losed its distal glucuronide and successfully prepared glycyrrihetic acid monoglucuronide (GAMG). Glycyrrihizic acid would lose a group of glucuronic acid by the hydrolysis of β-glucuronidase, and prepared GAMG which was a high intensity sweetner and has a higher biological activity. Immobilized enzyme technology means to immobilize biocatalyst such as enzyme or cell, that is to locate the biocatalyst in centain area. Immobilized enzyme could overcome the shortcomings of free enzyme, so it could be used in repetition. So it has been applied into many fields, such as food, chemical industry, medicine, chemical analysis, and so on. In this research, immobilized β-glucuronidase was used in the preparation of glycyrrhetic acid mono glucuronide. The immobilization method and its stability was studied, and the factors which had effects on the biotransformation were also studied. KEYWORDS: β-glucuronidase；Immobilization；Glycyrrhizic acid；Glycyrrihetic acid monoglucuronide； 目 录 摘 要 ABSTRACT 2 第1章 文献综述 1.1 甘草酸的研究进展 1.2 单葡萄糖醛酸甘草次酸的研究进展 1.2.1单葡萄糖醛酸甘草次酸的制备方法 1.2.2 单葡萄糖醛酸甘草次酸的应用 1.3 β-葡萄糖醛酸苷酶简介 1.3.1 高等动物组织中的β-GUS 1.3.2 软体动物消化液中的β-GUS 1.3.3 微生物中的β-GUS 1.4 固定化技术的研究概况 1.4.1 固定化技术的用途及发展前景 1.4.2 固定化的方法 第2章 材料和方法 2.1 试剂和仪器 2.1.1 试