壳低聚糖-海藻酸钠微胶囊的制备与表征.pdfVIP

中国组织工程研究与临床康复第13 眷第25 Jt!J 2009 - 06 - 18 出版 @束在吏基础实验 Joumal 0' Clinical Rehabilitative Tissue Engineering Research June 18, 2009 Vol.13, NO.25 壳低聚糖一海藻酸纳微胶囊的制备与表征** 杨明珠，田猛，李东旭，张云婷，孟令蝶，沈磊，孙树东，万昌秀 Preparation and peñormance of alginate-oligochitosan microcapsules Yang Ming-zhu, Tian Meng, Li Dong-xu , Zhang Yun-ting , Meng Ling-die, Shen Lei , Sun Shu-dong , Wan Chang呱』 Abstract College ofPo加W 。BJECTIVE: To Investigate the preparatlon conditions of the oligochitosan-alginate microcapsules and anal严e 伽emembrane Scien曲and thickness , mechanical strength and permeability so as to make a microcapsule with the best performance Englneering, Sichuan METHODS: The microcapsule was prepared by changing the pH value of the oligochitosan solution and reaction time. Inverted University, Chengdu 610065, Sichuan phase ∞ntrast microscope was used to observe and calculate the membrane thickness. Mechanical strength of microcapsule was Provin饵， China determined with a shaker and a pressure testing machine. Membrane permeability was estimated with bovine serum albumin (BSA) release profiles Yang Ming-zhu , RESULTS: Membrane thickness of the microcapsule was increased with the increase of the reaction time when the pH value of College of Polym町 Scienæand the oligochitosan solution reached 7. Under this condition , the compress modulus of the microcapsule had reached 5.12 Kpa after Engin回ring， Sichuan 10 minutes and none of them damaged after shaking for 6 hours. Protein release rate of the microcapsule decreased with the Unive陌ity， Chengdu increase of reaction time. When reaction for 10 minutes, protein release rate decreased with the increase of the ∞ncentration of 610065 , Sichuan Provin阻， China salt ions: however, protein