尖叶假龙胆中雏菊叶龙胆酮的分离、表征及抗菌活性研究.pdfVIP

第27 卷第 12 期 化学研究与应用 Vol. 27 ,No. 12 2015 年 12 月 Chemical Research and Application Dec. ,2015 文章编号:1∞4-1656(2015) 12-1811~6 Studies 00 Isolatioo ,characterizatioo ,and antibacterial activity of bellidifolio from gentianella acuta LV Li-juan 1 ,ZHU Hua-ling1 ,WANG Yan-fang2 ,JIANG Shuai2 ,ZHAO Wei 1 ,ZHAO Ya-chan 1 ,LI Min-hui2 * (υ1. Department of Basic 创Scclen缸encωe 厅，， 白1Tifian缸叫njin Ag伊ncu叫lt阳ur，时al Uni阳ver町B创由i让ty ， Tia电 2. Baotou Medical College ,Baotou 01ωω， China) Abs位act: Bellidifolin was isolated from Gentiarrella acuta ,and characterized by IR ,UV-vis spectra and X-ray diffraction analysis. It crystallizes in the triclinic lattice ,space group P-l with a=7. 侃但(14) Å,b=7. 5236 ( 15)Å,c= 10. 662(2) Å ， α=86.65(3)0 ， ß= 3 3 79.84(3)0 ， γ=84.96(3) 0 ,V =554.77 (1 9) Å ,Z=2 ,D. = 1. 642 glcm and F(创lO) = 284. Geometrical structure of bellidifolin was optimized by density functional th切可(DFT) using B3LYP method 明白6-31 g( d ,p ) as the basis set. 币le vibrational frequencies were calculated by the DFT/6-31g(d ,p)method and the results are nearly consistent with the observed frequencies. And 由e elec- tronic abωrption spectra were studied 明白白e time-dependent density functional theory(τD-DFT)and 由e calculated results 缸哩 m good 鸣reement with the coπ四，ponding experimental data. In addition ,the prelirninary antibacterial activities of bellidifolin against Staphyl∞∞cus aureus ,Escherichia coli ,Sal11Wnella enterica and Pser.uW11W阳aen伊nosa were investigated. Key words: bellidifolin; Gentianella acuta; characterization; DFT calculation; antibacterial activity 尖叶假龙胆中雏菊叶龙胆嗣的分离、