牛黄对小鼠口腔成纤维细胞功能的调节作用详解.PDF

生 物 工 程 学 报 Chin J Biotech 2009, March 25; 25(3): 448-451 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 组织工程与细胞培养 代剑平, 陈钧, 韩邦兴, 贝宇飞, 周晓坤 , 212013 : 探讨牛黄对原代小鼠口腔成纤维细胞功能的影响, 揭示其在溃疡愈合过程中的作用及机制。本试验采用 MTT 法、氯胺-T 法、明胶酶谱分析和酶联免疫反应测定了牛黄对小鼠口腔成纤维细胞增殖、胶原沉积、金属蛋白酶-2 、-9 活性和基质金属蛋白酶抑制因子-1 合成的影响。结果表明牛黄能显著抑制小鼠口腔成纤维细胞的增殖、胶原沉积和金 属蛋白酶-2 活性, 同时也极显著(P 0.01)抑制基质金属蛋白酶抑制因子-1 的产生。结果提示牛黄在溃疡愈合过程中不 具生肌作用, 可能通过抗炎促进溃疡愈合; 其抑制胶原合成的机制可能与极显著抑制基质金属蛋白酶抑制因子-1 有关。 : 胶原合成, 小鼠口腔成纤维细胞, 金属蛋白酶-2, -9, 基质金属蛋白酶抑制因子-1 Regulation of calculus bovis on the function of mice oral fibroblasts Jianping Dai, Jun Chen, Bangxing Han, Yufei Bei, and Xiaokun Zhou School of Food and Bioengineering, Jiangsu University , Zhenjiang 212013, China Abstract: To explore the influence of calculus bovis on the function of primary cultured mice oral fibroblasts, we determined the effects of calculus bovis on the fibroblast proliferation, collagen production, matrix metalloproteinases-2, -9 activities and tissue inhibitor of metalloproteinase-1 production by MTT assay, chloramine T method, gelatin zymography and enzyme-linked immunosorbent assays respectively. The results showed that calculus bovis could significantly inhibit the proliferation of fibroblasts and collagen synthesis in a concentration dependent manner, could significantly (P0.05) suppress matrix metalloproteinases-2 activity and very significantly (P0.01) inhibit the production of tissue inhibitor of metalloproteinase-1. In conclusion, the major function of calculus bovis in the process of ulcer healing is not to promote tissue regeneration,