反相色谱中如何选择缓冲液(国外英语资料).doc

反相色谱中如何选择缓冲液(国外英语资料) How to select the buffer in the reversed phase chromatography (2010-11-23 10:00:03). We label: buffer culture classification: analysis of professional - chromatography Statement: This article is seen elsewhere, quite good, reproduced here for everyone to see! Classification of compounds HPLC separated samples can be divided into neutral and ionic samples from their state in the separation system (or in the mobile phase). A neutral sample is a compound that cannot be dissociated under separate conditions, whereas an ionic compound can dissociate ions. In other words, an ionic sample is a compound of acids, bases and salts, and a neutral compound is a compound other than that. Are there good salt flow Reverse phase chromatography (RPC) separation of samples is mainly based on the polarity of the compounds to be separated. Under other conditions similar conditions (molecular weight and spatial structure), polar material retains little value. For the neutral sample depends on the polarity of the compound itself, but for ionic compounds can be adjusted by the dissociation degree of dissociation or whether to adjust its polarity, to adjust its retention, separating degree and effect on (general retention in advance will bring on the improvement of). In the case of a sample containing a few compounds, the use of a salt free mobile phase is generally feasible (regardless of whether all or part of the neutral compound is not considered). Because the separation of the compounds is small, the requirement of the separation degree is not very important, and the adjustment of the retention value can be realized by adjusting the elution capacity and flow velocity of the mobile phase. However, the use of salt free mobile phase often leads to trouble for the separation of multi component samples. At this point, it is necessary to try using a salt mobile phase, that is, to use buffers. Of course, if the compounds in the sample are all neutral compounds, the use