两种急性肺损伤动物模型的比较.pdfVIP

两种急性肺损伤(ALI)动物模型的比较 1 2 李守明 刘志勇 1 江西省儿童医院 2 江西省劳卫职防所实验动物中心 摘要 目的 研究两种急性肺损伤的动物模型 并探讨其在急性肺损伤研究中的比较医学 意义 方法 分别用一次性注射内毒素脂多糖 LPS S.D 大鼠急性肺损伤模型和用盲肠结 扎穿刺 CLP 法的豚鼠急性肺损伤模型 结合动脉血气分析 外周血白细胞计数 肺湿重 干重比值(W D)及肺组织病理观察 比较两种动物模型 结果 在LPS 模型中动物很快就 可以出现持续的低氧血症和明显的病理组织学改变,大剂量 LPS 注入数小时就会出现死亡 而在CLP 模型中动物的症状和表现缓慢出现 逐渐恶化 最后导致败血性休克 于2 3 天 左右死亡 结论 用盲肠结扎穿刺的方法制作豚鼠急性肺损伤动物模型较大鼠内毒素性休克 表现更似于人类的肠源性肺损伤 且症状缓慢发生 逐渐恶化 有利于观察和进行各种干预 关键词 呼吸窘迫综合症 肺损伤 动物模型 大鼠 豚鼠 中图分类号 文献标识码 A 文章编号 Two animal model of acute lung injury Li Shouming1 Liu Zhiyong2 1 Jiangxi Provincial Childrens Hospital Nanchang 330006 P R China 2 Jiangxi labor sanitation occupational disease prevention and treatment institute Nanchang 330006 P R China abstract objective To study two kinds of animal model of acute lung injury (ALI ) induced by lipopolysaccharide(LPS ) and cecal ligation –peferation(CLP )and their comparalogic significance . Methods In “LPS” animal model, the lipopolysaccharide LPS were given in gradually increasing dosage to S.D rats 2.5, 5 and 7.5 mg/kg respectively and in CLP animal model, the cecums of Guinea pigs had been ligated and peferated. Artery blood gases, peripheral blood white cell count, wet/dry weigh ratio of the lung were test, at the same time, pathologic morphometry of the lung were observed. Results The refractory hypoxemia syndromes were developed rapidly in the LPS model and the animals were died of septic shock within hours, compared with it in CLP model which were developed gradually and the animals