13 182-186 工艺技术 5-8 纤维素酶酶解玉米秸秆新型蒸煮浆的工艺研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.2 纤维素酶酶解玉米秸秆新型蒸煮浆的工艺研究 张鹤，佟心洁，庞春生，谢土均 （华南理工大学轻工与食品学院，广东广州510640 ） 摘要：本文以玉米秸秆活性氧固体碱蒸煮浆（木素脱除率82.1% ）为原料，用国产纤维素酶（酶活为13.94 FPU/100 mL ）进行酶解试验，探索酶解最佳工艺条件。结果显示：底物固液比为0.47% （m/V ），酶解pH值为4.70 ，酶解温度为50 ℃，酶解时间为72 h 时酶解液还原糖含量最大，即糖得率最大，达59.41%。本研究也进行玉米秸秆经不同方法预处理后的酶解实验，结果显示其 纤维素酶解效果顺序为：活性氧固体碱蒸煮浆＞硫酸盐浆＞机械粉碎＞未预处理玉米秸秆。 关键词：玉米秸秆浆；活性氧；固体碱；纤维素酶；酶解 文章篇号：1673-9078(2012)2-182-186 Research of Enzymatic Hydrolysis of Corn Stalk Pulp with New Cooking Method ZHANG He, TONG Xin-jie, PANG Chun-sheng, XIE Tu-jun (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp (removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase (enzyme activity: 13.94 FPU/100ml). The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47% (m/V), pH value of 4.70，temperature of 50℃ and enzymatic reaction time of 72 h. The highest yield of sugar was 59.41% under the optimum process conditions. The paper also presented the different enzymolysis of corn stalk pretreated by different methods, and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base corn stalk pulp from alkaline cooking corn stalk pretreated by mechanical crushing raw corn stalk. Key words: corn stalk pulp; active oxygen; solid alkali; cellulase; enzymatic hydrolysis. 182 伴随全球能源与环境问题的日益突出，低 焚烧带来