甘蓝自交不亲和信号传导元件ARC1的体外表达和其和SRK相互作用验证.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(7): 1202−1208 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.01202 甘蓝自交不亲和信号传导元件ARC1 的体外表达及其与SRK 相互作 用验证 牛 义1 王志敏1 高启国1 宋 明1 王小佳1,* 朱利泉2,* 1 ; 2 , 400716 : ARC1 SRK , E1 , RT-PCR ARC1 , ARC1 pET43.1a-ARC1, BL21, SDSpET43.1a-ARC1 6×His Ni+, ARC1 SRK , ARC1 SRK , ARC1 SRK ARC1 : ; ; S-(SRK); 1 (ARC1) Expression of ARC1 in Vitro and Test of Interaction between ARC1 and SRK from Brassica oleracea L. in Signal Transduction Pathway of Self-Incompati- bility 1 1 1 1 1,* 2,* NIU Yi , WANG Zhi-Min , GAO Qi-Guo , SONG Ming , WANG Xiao-Jia , and ZHU Li-Quan 1 Key Laboratory in Olericulture of Chongqing, Southwest University, 2 Plant Physiology and Biochemistry Laboratory of Southwest University, Chongqing 400716, China Abstract: Self-incompatibility (SI) is a widespread mechanism in flowering plants that prevents inbreeding and promotes out- crossing. Self-pollen recognition relies on the products of genes located at the S (self-incompatibility) locus. Significant progress has been made in understanding molecular interactions allowing stigmatic cells to recognize and reject self-pollen in Brassica during the past decades. Thus, the male and female determinants responsible for the self-incompatibility (SI) response have been identified. The structural features of these molecules strongly suggest that SI response is triggered by a ligand