LPS和TNFα对肺微血管内皮细胞内游离钙的影响论文.docVIP

　　LPS和TNFα对肺微血管内皮细胞内游离钙的影响论文 肖贞良，全燕，李福祥，钱桂生 【摘要】 目的 探讨脂多糖(LPS)、肿瘤坏死因子α(TNFα)对大鼠肺微血管内皮细胞(RPMVEC)内游离钙浓度(Ca2+i)的影响。方法 将细胞接种于盖玻片上，细胞单层汇合后进行实验。实验分组：LPS组(用10μg/ml LPS)，LPS+山莨菪碱组(用10μg/ml LPS+10μg/ml山莨菪碱)，TNFα组(用5000u/mlTNFα)，TNFα+山莨菪碱组(用5000u/ml TNFα+10μg/ml山莨菪碱).freelin后按照试剂盒提供的方法，并参照文献测定Ca2+i。结果 与正常对照组比较，LPS组、LPS+山莨菪碱组15min和90min两个时相点Ca2+i均显著升高(P 0.01);LPS组与LPS+山莨菪碱组比较，15min和90min两个时相点Ca2+i无明显差异(P 0.05)。同样，与正常对照组比较，TNFα组、TNFα+山莨菪碱组15min和90min两个时相点Ca2+i均显著升高(P 0.01);TNFα组与TNFα+山莨菪碱组比较，15min和90min两个时相点Ca2+i无明显差异(P 0.05)。结论 LPS、TNFα作用可使RPMVEC Ca2+i显著升高，山莨菪碱对LPS、TNFα诱导的RPMVEC Ca2+i升高无显著抑制作用;LPS、TNFα引起的Ca2+i浓度升高与β-AR介导的信号转导通路无关;Ca2+i升高可能参与了G蛋白偶联受激酶的活性调节。 【关键词】 内皮细胞;游离钙;脂多糖;肿瘤坏死因子α Abstract Objective To investigate the effects of lipopolysaccharide (LPS) and tumor necrosis factor α (TNF α) on intracellular free calcium (Ca2+i) of rat pulmonary microvascular endothelial cells (RPMVEC) and the relationship betount of cells s; experiment onolayer confluented; group division in the experiment l of LPS added), LPS+anisodamine group (10μg/ml of LPS and 10μg/ml of anisodamine added), TNFα group (5000μ/ml of TNFα added), TNFα+anisodamine group (5000μ/ml of TNFα and 10μg/ml of anisodamine added) and normal control group (equal amount of diluent added), and each group had 4 samples. Ca2+i. In each group easured at the time point of 15 and 90 minute ethod from reagent kit instruction and literature.Result Ca2+i in LPS group and LPS+anisodamine group at the time point of 15 and 90 minutes al control group (P 0.01), but there existed no significant difference of Ca2+i betine group at the same time points (P 0.01); Ca2+i in TNFα group and TNFα+anisodamine group at the time point of 15 and 90 minute al control group (P 0.01) but there ine group at the time point of 15 and 90 minute.Conclusions Both LPS and TNFα can lead to the significant increase of Ca2+i in RPMVEC; the increase of Ca2+i is not related to the signal transduction pathediated by beta adrenoceptor lipopolysaccharide tumo