黄连属部分药用植物遗传多样性及亲缘关系SCoT探析.docVIP

黄连属部分药用植物遗传多样性及亲缘关系SCoT探析 　　[摘要]采用SCoT分子标记分析4种黄连属药用植物的遗传多样性和亲缘关系，并用POPGENE计算遗传参数，TREECONW软件进行聚类分析。结果表明：28条引物共检测到434条带，其中多态性条带430条；各遗传参数均表明黄连属药用植物种间的遗传多样性极为丰富（种间PPB=99.1%，Na=1.990 6，Ne=1.329 3，H=0.212 2，I=0.337 8），但种内遗传多样性较低（种内PPB=16.8%，Na=1.168 2，Ne=1.073 0，H=0.043 7，I=0.067 7）；?z传分化系数Gst=0.794 0，说明79.4%的遗传变异来源于种间；同种黄连属药用植物的不同植株均聚在一起，证实SCoT分子标记可作为黄连属药用植物亲缘关系研究的有效方法之一 [关键词]黄连属； SCoT； 药用植物； 亲缘关系 [Abstract]The genetic diversity and genetic relationship among four medicinal species of Coptis were detected by the approach of sequence-related amplified polymorphism （SCoT）. The associated genetic parameters were calculated by POPGENE1.31. The systematic diagram of genetic relationship were clustered by TREECONW. The results showed that a total of 434 bands were produced by using 28 primers， of which 430 were polymorphic loci. There was a high level of genetic diversity among species （PPB=99.1%，Na=1.990 6，Ne=1.329 3，H=0.212 2，I=0.337 8）. However， genetic diversity was lower within species， the average of genetic parameters wasPPB=16.8%，Na=1.168 2， Ne=1.073 0，H=0.043 7，I=0.067 7. The Nei′s genetic differentiation coefficient was 0.794 0， that indicated that most of the genetic variation existed among species. By clustering analysis， different individuals gathered in the same group. The results confirmed that SCoT marker can be used as one of the effective methods to reveal the genetic diversity and relationship among medicinal species of Coptis. [Key words]SCoT； Coptis； medicinal plant； genetic relationship 黄连为名贵常用中药之一，始载于《神农本草经》，在我国已有2 000多年的药用历史，具有清热燥湿，泻火解毒的功效。我国分布的黄连属植物中黄连Coptis chinensis Franch.、三角叶黄连C. deltoidea C.Y.Cheng et Hsiao.及云南黄连C. teetoides C.Y.Cheng.是《中国药典》各版本收录药材黄连的基原植物 [2]。此外，分布于四川峨眉、雅安与洪雅地区的峨嵋野连C. omeiemsis （Chen） C.Y.Cheng.、西藏南部昌都地区的西藏黄连C. teeta Wall.及四川马边一带的线萼黄连C. linearisepala T.Z.Wang et C.K.hsieh.等多种栽培或野生黄连属植物的根茎作为药用。《日本药局方》除收载了《中国药典》收录药材黄连的基原植物外，还收载了日本黄连C.japonica Makino.。因此，理清黄连属法定用种及地方用种的亲缘关系，准确进行基原鉴别对确保黄连的临床用药安全具有重要意义 黄连属药用植物亲缘关系的研究及鉴别早