中药丹参酮对复合痴呆大鼠海马内诱导型一氧化氮合酶和乙酰胆碱酯酶的影响论文.docVIP

　　中药丹参酮对复合痴呆大鼠海马内诱导型一氧化氮合酶和乙酰胆碱酯酶的影响论文 【摘要】 目的探讨丹参酮对D-半乳糖- Aβ1-40致复合痴呆模型大鼠海马内诱导型一氧化氮合酶（inducible nitric oxide synthase，iNOS）和乙酰胆碱酯酶（acetylcholinesterase，AChE）表达的影响。方法① 实验动物随机分成3组：AD模型组：采用1.25% D-半乳糖腹腔注射致衰老结合双侧海马齿状回背侧微量注射凝聚态Aβ1-40复合造模方法，拟建立AD样学习记忆功能障碍的痴呆动物模型；丹参酮治疗组：AD大鼠造模24 h后给予丹参酮〔50 mg/(kg·d)〕；溶媒对照组：与AD大鼠同法注射等量生理盐水。② 采用免疫组织化学和酶组织化学方法，分别观察大鼠海马内iNOS和AChE表达的变化。结果①模型组AChE阳性纤维受损，光密度下降（含阳性面积百分比和光密度）；iNOS-ir细胞数显著升高，平均光密度增强；与对照组比较差异极显著（P＜0.01）。②丹参酮处理后，iNOS-ir细胞数明显减少.freelpus of dementia model rats induced by D-galactose bination ental animals ly divided into three groups. In AD model group 1.25%D-galactose in bination icroinjection of 10 μg incubated amyloid β-peptide 1-40 (Aβ1-40) into the dorsal blade of the dentale gyrus in the hippocampus of rat. In tanshinone therapeutic group,after establishment of rat AD model for 24 h , tanshinone〔50 mg/(kg·d)〕e method as AD rats but received injection of the same physiological saline. ② The expression of iNOS and AChE in rat hippocampus munohistochemical and histochemical methods .Results①AChE positive nervous fibers, optical density of AChE positive nervous fibers of AD rats in the hippocampus pus ent, iNOS -ir cell count and the optical density odel group.ConclusionMechanism of tanshinone to improve learning and memory impairment in dementia model rats induced by D-galactose in bination ight be related to protection of cholinergic nerves in brain and decrease of iNOS expression in hippocampus . Key yloid peptide protein (Aβ); Inducible nitric oxide synthase（iNOS）； Acetylcholinesterase（AChE） ; Rats 老人常见的痴呆病因是阿尔茨海默病（Alzheimer’s disease, AD）［1］，而AD的病因、发病机制尚未完全清楚。AD患者大脑内主要变化的神经递质是乙酰胆碱（acetylcholine ，ACh），一氧化氮（nitric oxide, NO）及诱导型一氧化氮合酶（inductive nitric oxide synthase, iNOS）［2～4］ 。有报道，丹参酮ⅡA和ⅡB能减少小鼠短暂的（急性）局灶性脑缺血的脑梗死灶体积，并可能有神经保护作用［5］，还能改善缺血再灌注损伤小鼠和东莨菪碱（抗胆碱药）诱发的空间行为缺陷小鼠的学习、记忆障碍［6］。但就丹参酮对D-半乳糖- Aβ1-40诱发复合痴呆（记忆障碍）病变大鼠的作用未见报道。因此，本研究采用腹腔注射1.25%D-半乳糖致衰老加双侧海马微量注射凝聚态Aβ1-40诱发AD样复合痴呆（记忆障碍）大鼠模型，以观察海马内ACh的降解酶乙酰胆碱酯酶（acetyl cholinesterase, ACh