人参Rb组皂苷对压力负荷性心肌肥厚大鼠心室重构的影响及其作用机制论文.docVIP

　　人参Rb组皂苷对压力负荷性心肌肥厚大鼠心室重构的影响及其作用机制论文 王天晓，于小风，曲绍春，徐华丽，韩冬，睢大员 【摘要】 目的观察人参Rb组皂苷（G-Rb）对压力负荷性心肌肥厚大鼠心室重构的影响及其作用机制。方法结扎大鼠腹主动脉建立压力超负荷性心室重构模型。G-Rb按25，50，100 mg/（kg·d）给大鼠连续灌胃6周.freelodeling in rats yocardium and its mechanism. MethodsThe model of pressure-loading ventricular remodeling inal aorta in rats. After 6 ent by G-Rb (g/kg/d i.g for 6 yocardial morphological and hemodynamic parameters ined. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in serum, and the contents of prostacycline (PGI2), thromboxane A2 (TXA2), endothelium (ET) and angiotensinⅡ (AngⅡ) in plasma ined. ResultsG-Rb could significantly decrease the ventricular odeling rats, significantly heighten systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP), lo and significantly increased the activity of SOD in serum. In addition, G-Rb could decline the contents of ET, AngII and TXA2 in plasma a and PGI2 /TXA2 ratio. pared odel group, there odeling through improving systolic and diastolic function of cor sinistrum in ventricular remodeling rats，increasing anti-oxidase activity, reducing the damage of free radicals and vasoactive substance in myocardium and correcting disequilibrium of PGI2/TXA2 in ventricular remodeling etc. Key odeling; Myocardial morphology; Hemodynamics; Biochemics of blood; Rats 人参Rb组皂苷（Ginsenoside-Rb，G-Rb）系从五加科人参属植物西洋参Panax quinquefolium Linn茎叶及根提取的总皂苷中分离得到的有效部位群，保证了人参皂苷Rb在组分（包括Rb1、Rb2和Rb3）及含量上的完整性。本室前期研究表明，G-Rb能明显缩小急性心肌梗塞犬心肌梗塞面积，降低血清磷酸肌酸激酶及乳酸脱氢酶活性，并明显降低血清LPO含量，提高SOD活性［1］；可明显降低实验性心肌梗塞大鼠血液黏度和游离脂肪酸水平［2］；亦能通过降低心肌耗氧量，改善冠脉循环、抗自由基氧化损伤及增加心肌营养性血流量等环节对缺血心肌产生保护作用［3～5］。现已按治疗胸痹心痛（冠心病心绞痛）的中药五类新药（双参素胶囊）开发。为了明确G-Rb长期给药是否具有防治心肌梗塞后心室重构的作用，本实验采用结扎大鼠腹主动脉建立压力超负荷性心室重构模型，通过左心室功能和心脏形态学等参数观察G-Rb对大鼠实验性心室重构的防治作用，并初步探讨其对抗心室重构的作用机制。 1 材料与方法 1.1 动物 清洁级DA）及超氧物歧化酶（SOD）测定试剂盒均由南京建成生物研究所提供。 1.3 心室重构模型制备［6］ 采用腹主动脉缩窄法制备压力负荷性心室重构模型。常规消毒腹部皮肤，以3%戊巴比妥钠30 mg/kg腹腔注射麻醉，除毛，取上腹正中切口打开腹腔，分离左肾动脉起始部上段腹主动脉约10 mm，将一直径约0.6 mm的光滑针头平行置于该段腹主动脉上，以4.0丝线将腹主动脉连同针头一起结扎