人参茎叶皂苷对热损伤大鼠不同脏器糖皮质激素受体的影响论文.docVIP

　　人参茎叶皂苷对热损伤大鼠不同脏器糖皮质激素受体的影响论文 李敏，凌昌全，黄雪强，沈志雷 【关键词】 人参皂苷 ［摘要］ 目的：观察人参茎叶皂苷（ginsenosides, GSS）对热损伤大鼠不同脏器糖皮质激素受体（glucocorticoid receptor, GR）的影响，并探讨其作用机制。方法：雄性SD大鼠随机分为：（1）正常对照组，室温下饲养，蒸馏水灌胃；（2）GSS治疗组，室温下饲养.freelRNA的水平；放射免疫法测定血浆促肾上腺皮质激素（adrenocorticotropin, ACTH）和皮质酮（corticosterone, CS）的浓度。结果：热损伤模型GSS治疗组大鼠脑、肺和肝细胞液GR结合活性以及脑和肝细胞液GR mRNA表达水平均明显高于单纯热损伤模型组（P＜0.05或P＜0.01）；热损伤模型GSS治疗组大鼠血浆ACTH和CS浓度与单纯热损伤模型组比较则无明显差异。结论：GSS可缓解热损伤大鼠不同脏器GR结合活性的下降幅度，其作用机制可能与促进GR mRNA的表达有关。 ［关键词］ 人参皂苷; 人参茎叶; 糖皮质激素受体; 热损伤; 大鼠 Effects of ginsenosides extracted from ginseng stem and leaves on glucocorticoid receptor in different viscera in heatdamaged rats ABSTRACT Objective: To evaluate the effects of ginsenosides (GSS) extracted from ginseng stem and leaves on glucocorticoid receptor (GR) in different viscera in heatdamaged rats, and to find out its action mechanism. Methods: Thirtytale SD rats ental group, and fed 2 mg/d GSS and equalquantity of distilled us, lung and liver cytosols in rats RNA in brain and liver cytosols ined by reverse transcriptionpolymerase chain reaction (RTPCR) assay. Plasma adrenocorticotropin (ACTH) and corticosterone (CS) concentrations ined by radioimmunoassay. Results: The binding activities of GR in brain, lung and liver cytosols, and the expression levels of GR mRNA in brain and liver cytosols aged rats than those in the untreated heatdamaged rats (P＜0.05 or P＜0.01). There a concentrations of ACTH and CS betaged rats and the untreated heatdamaged rats. Conclusion: GSS can lessen the descending degree of the binding activity of GR in brain, thymus, lung and liver cytosols,.freelay be related to improvment of the expression of GR mRNA. KEY MLV Reverse Transcriptase（美国Gibco BRL公司产品），37 ℃保温60 min。90 ℃处理5 min，冰上冷却，室温离心将溶液收集至管底。PCR扩增，引物由上海博彩生物科技有限公司合成，上游5’ AGG CCG GTC AGT GTT TTC TA 3’，下游5’ AGG GGT GAG CTG TGG TAA TG 3’，扩增得GR mRNA长度433 bp，每个反应用2.5 μl 10×PCR buffer（mol/L MgCl2），19.25 μl H2O，0.5 μl 10 mmol/L 4×dNTP mix，0.25 μl iNosUP，0.25 μl iNosDol PCR薄壁管里，每管23 μl。内参βactin