鲈脾肿大症的病原及细胞病理学的初步研究摘要.PDFVIP

272 Vol.27, No. 2 2003 4 JOURNAL OFFISHERIES OF CHINA Apr., 2003 :1000- 0615(2003)02- 0158- 05 王国良, 金 珊, 陈惠群 ( , 315211) :,50 ,, ,, 180~ 220nm, (Iridoviridae) ,, , 50 :; ;; :S941.41 : A Pathogen and cytopathology of splenomegaly disease in Lateolabrax j ap onicus WANG Guo-liang, JIN Shan, CHEN Hui-qun ( Dep artment of Oceanography and Fisheries, Ningbo University, Ningbo 315211, China) Abstract : Thispaper first reportsthe splenomegaly disease of seawater-cage cultured Lateolabraxj aponic s. The main symptom of diseased fish is splenomegaly. Mortality rate of diseased fish reaches 50 . Neither parasites were found on body surface, gill filaments and other organs by microscope exmination, nor pathogenic bacteria isolatedfrom internal organs. However, under an electron microscope, a lot of virusparticleswere observed in spleenand liver of the diseased fish. The virus was a non-envelope with hexagon in section and about 180- 220nm in diameter. The virus was assigned to Iridoviridae. Cytopathological changes showed that the cells swelling and ultrastructure broke with large area vacuoles in cytoplasm. A lot of distinctive lymphocytes were found in blood cells, which occupies 50 of the total number of the lymphocytes. Key words: Lateolabraxj aponic s; splenomegaly disease; pathogen; ultrastructure (Lateolabraxj aponic s) , , 40 ,,, , :2002-09-05 :(396269) :( 1955- ) , ,,,Tel:0574-E-mail:wanggl@nbip.net.cn 2 : 159 , 1 20008, 10~ 15cm, 30~ 50g, 2 2. 1 2.2 ,PGY2216E TCBS ,25e 24h 2.3