阻断血管内皮生长因子C促进角膜移植后新生淋巴管和血管的“分离生长”.doc

Lymphatic vessels growing apart from blood vessels in transplanted corneas after the blockade of vascular endothelial growth factor C Ye Hui1, Yan Hao2, Zhong Lei1, Wang Tao1, Deng Juan1, Ling Shi-qi1 1 Department of Ophthalmology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China 2 Department of Ophthalmology, Nanshan Hospital of Guangdong Medical College, Shenzhen 510080, Guangdong Province, China Cite this article: Ye H, Yan H, Zhong L, Wang T, Deng J, Ling SQ. Lymphatic vessels growing apart from blood vessels in transplanted corneas after the blockade of vascular endothelial growth factor C. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(33): 4940-4948. DOI:10.3969/j.issn.2095-4344.2016.33.011 ORCID: 0000-0002-4948-9236(Ling Shi-qi) Abstract BACKGROUND: Corneal lymphangiogenesis is beneficial to the transport of corneal antigenic materials, and accelerates the process of antigen presentation, thereby playing an important role in corneal immunity. However, due to the parallel outgrowth of corneal blood and lymphatic vessels in transplanted corneas, it is often difficult to accurately evaluate the role of corneal lymphatic vessels in allograft rejection. OBJECTIVE: To explore the development of corneal lymphangiogenesis and angiogenesis in transplanted rat corneas after the blockade of vascular endothelial growth factor C (VEGF-C). METHODS: 130 rats used to establish corneal allogenic transplantation models were equally randomized into two groups: the anti-VEGF-C group and the control group. VEGF-C was blocked in the anti-VEGF-C group by intraperitoneal injection of neutralizing monoclonal anti-VEGF-C antibody every other day for 2 consecutive weeks. Meanwhile, rats in control groups received intraperitoneal injections of saline. Corneal angiogenesis and lymphangiogenesis were characterized using whole mount immunofluorescence, and the immune rejection of the grafts was evaluated by scoring the rejection index (