四川牡丹种子浸提液内源抑制物活性初探.pdfVIP

园 艺 学 报 2012 ，39 （2 ）：370 –374 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 四川牡丹种子浸提液内源抑制物活性初探 宋会兴，刘光立，高素萍，陈其兵* （四川农业大学风景园林学院，成都 611130 ） 摘 要：通过研究四川牡丹（Paeonia decomposita Hand.-Mazz. ）种皮、胚乳水浸提物对白菜种子萌 发、幼苗生长及保护酶活性的影响，探讨四川牡丹种子内源抑制物质的活性。结果发现，四川牡丹种皮、 胚乳中含有抑制白菜种子萌发及幼苗生长的物质，随着该物质浓度的增加，抑制作用更为显著；相同浓 度的胚乳浸提液对白菜种子萌发及幼苗生长的抑制作用显著高于种皮浸提液。胚乳浸提液能够直接抑制 白菜幼苗过氧化氢酶（CAT ）和过氧化物酶（POD ）活性，间接影响超氧化物歧化酶（SOD ）活性。结果 表明：四川牡丹种子内源抑制物质的存在，可能是四川牡丹种子休眠的重要原因；四川牡丹种子浸提物 可能通过影响保护酶的活性进而影响白菜幼苗的正常生长。 关键词：四川牡丹；休眠；白菜；保护酶；抑制作用 中图分类号：S 685.11 文献标识码：A 文章编号：0513-353X （2012 ）02-0370-05 Effects of Crude Extracts of Paeonia decomposita Seeds on Germination and Activities of Antioxidant Enzyme of Brassica pekinensis * SONG Hui-xing，LIU Guang-li ，GAO Su-ping，and CHEN Qi-bing （School of Landscape Architecture ，Sichuan Agricultural University ，Chengdu 611130，China） Abstract ：The effects of water crude extracts of the seed coat and endosperm of Paeonia decomposita on the seed germination，seedling growth and antioxidant enzymes of Brassica pekinensis were examined to understand the cause of seed dormancy of P. decomposita Hand.-Mazz. The results showed that the seed，especially the endosperm tissue of P . decomposita ，contained substances that strongly suppressed seed germination. The crude extract of endosperm of P . decomposita ，which significantly reduced the activities of catalase and peroxidase ，and indirectly affected the activity of superoxide dismutase ，showed a more significant inhibition than that of seed coat at the same dose. It was concluded that the presence of inhibitory substances is one of important causes of seed dorma