中国原产果梅自交亲和变异品种花粉决定基因_em_SFB_em_的插入突变.pdfVIP

园 艺 学 报 2012 ，39 （3 ）：413 –460 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 中国原产果梅自交亲和变异品种花粉决定基因 SFB 的插入突变 * * 王培培，侍 婷，高志红 ，章 镇 ，庄维兵 （南京农业大学园艺学院，南京 210095 ） 摘 要：果梅是配子体自交不亲和树种，但在长期进化过程中也出现了自交亲和品种。以果梅典型 的自交不亲和品种‘南高’和自交亲和品种‘甲州小梅’为对照，采用田间自交授粉试验对原产于中国 的‘四川白梅’和‘长农 17’两个果梅品种进行了自交亲和性鉴定，结果表明：其自花授粉坐果率分别 为 30.73%和 16.27%，属于自交亲和品种。进一步通过 AS-PCR 分析发现花粉 SFB 基因存在一段插入序列， 造成了分子水平的基因突变。推测该基因的突变使‘四川白梅’和‘长农 17’SFB 功能发生改变，从而 自交亲和。 关键词：果梅；自交亲和性；鉴定；AS-PCR 中图分类号：S 662.4 文献标识码：A 文章编号：0513-353X （2012 ）03-0453-08 Insertion Mutation of Pollen SFB Gene in Self-compatibility of Japanese Apricot Cultivars Native to China * * WANG Pei-pei ，SHI Ting，GAO Zhi-hong ，ZHANG Zhen ，and ZHUANG Wei-bing （College of Horticulture，Nanjing Agricultural University ，Nanjing 210095 ，China） Abstract ：Japanese apricot （Prunus mume Sieb. et Zucc ）is a fruit crop with gametophytic self-incompatibility. Some self-compatible varieties appeared during long-term evolution. In this study ，a typical self-incompatibility cultivar ‘Nanko ’and self-compatible cultivar ‘Koshinoume ’of Japanese apricot were taken as controls. We identified the self-compatibility of two cultivars ‘Sichuan Baimei ’and ‘Changnong 17 ’native to China through field self-pollination test ，the results demonstrated that the rate of fruiting set were 30.73% and 16.27%，respectively. Further analysis of AS-PCR showed that there were insertion mutation in pollen SFB genes of the two cultivars ，which possibly resulted in self-compatibility of these two cultivars. Key words ：Japanese apricot ；self-compatibility；identification ；AS-PCR 自交不亲和性（Self-incompatibility，SI ）是被子