大葱细胞质雄性不育基因的SCAR 标记开发.pdfVIP

园 艺 学 报 2013 ，40 （7 ）：1382 –1388 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 大葱细胞质雄性不育基因的 SCAR 标记开发 高莉敏，董 飞，霍雨猛，刘冰江，缪 军，陈运起* （山东省农业科学院蔬菜研究所，山东省设施蔬菜生物学重点实验室，国家蔬菜改良中心山东分中心，济南 250100 ） 摘 要：以大葱（Allium fistulosum L. ）雄性不育系和保持系为试材，开发了一个能够鉴别细胞质雄 性不育类型的 SCAR 标记。此标记在大葱雄性不育系（S 型细胞质）中扩增出１条 607 bp 片段，而在保 持系（N 型细胞质）中没有扩增出此片段，将此片段命名为 S607。对 5 组不同遗传背景的不育系和相应 保持系，以及 4 份杂交组合进行验证，SCAR 标记 S607 鉴定结果与实际细胞质类型完全相符。 关键词：大葱；细胞质；雄性不育；SCAR 标记 中图分类号：S 633.1 文献标志码：A 文章编号：0513-353X （2013 ）07-1382-07 Development of SCAR Marker Identifying the Cytoplasmic Male Sterility Gene in Bunching Onion （Allium fistulosum L. ） * GAO Li-min，DONG Fei ，HUO Yu-meng ，LIU Bing-jiang ，MIAO Jun ，and CHEN Yun-qi （Vegetable Research Institute of Shandong Academy of Agricultural Sciences，Key Laboratory for Biology of Greenhouse ， Vegetable of Shandong Province，National Improvement Center for Vegetable ，Shandong Branch ，Jinan 250100 ，China） Abstract ：Cytoplasmic genetic male-sterility （CMS ）is used to produce hybrid bunching onion （Allium fistulosum L. ）seeds worldwide. In this paper，we developed one SCAR marker using male sterile line（980238A ）and maintainer line （980238B ）as sterile and fertile gene pool for the target band screening. This marker was 607 bp in size and designated as S607 which was specifically amplified in the male sterile line，and it could distinguish two cytoplasm types CMS-S （Sterile type）and CMS-N （Normal type ）. The SCAR marker was verified in five male sterile lines，five maintainer lines，and four cross combinations， all of these valuations were in accord with their cytoplasmic type （S or N