越瓜果实发育过程中糖的变化与相关酶活性的关系.pdfVIP

中国蔬菜　2013（6）：66-70 CHINA VEGETABLES 越瓜果实发育过程中糖的变化与相关酶活性 的关系 田红梅　方　凌　王　艳　王明霞　江海坤　严从生　张其安* （安徽省农业科学院园艺研究所，安徽合肥 230031） 摘　要： 以从各地搜集的 6 个越瓜品种为试材，对越瓜果实糖含量及蔗糖代谢相关酶进行了研究。结 果表明：不同越瓜品种之间糖含量差异较大，凤台越瓜、田集越瓜、刘集越瓜 3 个品种可溶性固形物含量 及糖含量较高；随着果实的发育蔗糖磷酸合成酶活性呈递增趋势，且在果实各个发育期田集越瓜的蔗糖磷 酸合成酶活性均最高；而转化酶活性则随着果实发育呈下降趋势，凤台越瓜和田集越瓜的酸性转化酶及中 性转化酶活性在各个发育期均最低；中性转化酶在毛集越瓜、潘集越瓜、青皮越瓜、刘集越瓜中相对较高， 且随着果实发育活性下降，至果实成熟时活性达到最小值；除刘集越瓜外，其他品种的蔗糖代谢酶活性在 果实不同发育期均没有明显变化。以上结果表明，不同越瓜品种蔗糖含量差异是由蔗糖磷酸合成酶、酸性 转化酶和中性转化酶活性共同决定的，其中酸性转化酶在越瓜蔗糖积累中占主导地位。 关键词：越瓜；蔗糖；蔗糖磷酸合成酶；酸性转化酶 中图分类号：S652.9　　文献标识码：A　　 文章编号： 1000-6346（2013）08-0066-05 Relationship between Sugar Content Change and Interrelated Enzyme Activity in Crisp Melon Fruit Development Process TIAN Hong-mei，FANG Ling，WANG Yan，WANG Ming-xia，JIANG Hai-kun，YAN Cong- * sheng，ZHANG Qi-an Institute of Horticulture Anhui Academy of Agricultural Sciences Hefei 230031 Anhui China （ ， ， ， ， ） Abstract ：We collected 6 crisp melon（ Cucumis melo L. var. conomon Makino）varieties as experiment materials and studied the sucrose content of crisp melon fruit and sucrose metabolism enzymes．The results showed that there were big differences in sugar contents of different crisp melon varieties．The soluble solid contents and sugar contents of 3 varieties‘Fengtaiyuegua’，‘Tianjiyuegua’， ‘Liujiyuegua’were higher．The sucrose phosphate synthase activity became higher and higher as the fruit grew and matured，and the enzyme activity was the highest in‘Tianjiyuegua’．While the invertase activity became lower and lower along with the fruit development．The activities of acid invertase and neutral invertase were the lowest in‘Fengtaiyuegua’and‘Tianjiyuegua’during various development stages．The activities of neutral invertase were high in‘Maojiyuegua’，‘Panjiyuegua’，‘Qingpiyuegua’， 收稿日期：2012-10-19；接受日期：2013-01-14 基金项目： 国家西甜瓜产业技