呼吸氧气引起氧化应激的研究.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2012, Article ID 381763, 5 pages doi:10.1155/2012/381763 Research Article Oxygen Concentration-Dependent Oxidative Stress Levels in Rats Fumiko Nagatomo,1 Hidemi Fujino,2 Hiroyo Kondo,3 and Akihiko Ishihara1 1 Laboratory of Cell Biology and Life Science, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan 2 Department of Rehabilitation Science, Kobe University Graduate School of Health Sciences, Kobe 654-0142, Japan 3 Department of Food Sciences and Nutrition, Nagoya Women’s University, Nagoya 467-8610, Japan Correspondence should be addressed to Akihiko Ishihara, ishihara.akihiko.8s@kyoto-u.ac.jp Received 19 April 2012; Revised 5 August 2012; Accepted 8 August 2012 Academic Editor: Jose Luis Martin-Ventura Copyright © 2012 Fumiko Nagatomo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. We determined derivatives of reactive oxygen metabolites (dROMs) as an index of oxidative stress level (oxidant capacity) and biochemical antioxidant potential (BAP) as an index of antioxidant capacity in rats exposed to different oxygen concentrations. Methods. Male Wistar rats were exposed to 14.4%, 20.9%, 35.5%, 39.8%, 62.5%, and 82.2% oxygen at 1 atmosphere absolute for 24 h. Serum levels of dROMs and BAP were examined by using a free radical and antioxidant potential determination device. The morphological characteristics of red blood cells were examined by phase contrast microscopy. Results. There were no differences in the levels of dROMs in rats exposed to 14.4%, 20.