孤束核蛋白酪氨酸激酶对外周化学感受性反射呼吸作用的影响.pdfVIP

Acta Physiologica Sinica, June 25, 2005, 57 (3): 395-399 395 * , 250012 (protein tyrosine kinase, PTK) 1 0% (10% O 90% N )(nucleus tractus solitarius, NTS) 2 2 genistein daidzein AMPA CNQX NTS CNQX genistein daidzein NTS CNQX genistein CNQX genistein NTS NTS AMPA PTK Q473; R332.2 Effects of protein tyrosine kinase wihin the brainstem nucleus tractus solitarius on the ventilatory responses of peripheral chemoreflex LI Yan-Chun, WANG Hui, CAO Ying, TANG Di, WANG Gui-Min, YU Shu-Yan, SONG Gang, ZHANG Heng* Institute of Physiology, School of Medicine, Shandong University, Jinan 250012, China Abstract: The aim of the present study was to observe whether protein tyrosine kinase (PTK) within the nucleus tractus solitarius(NTS) was involved in the regulation of ventilatory responses of peripheral chemoreflex. The experiments were performed on anesthetized, immobilized and artificially ventilated rabbits. Peripheral chemoreflex was elicited by ventilating the animal with 10% O2-balance 90% N . Changes in the peak amplitude and frequency of integrated phrenic nerve activity were observed. The ventilatory responses of 2 peripheral chemoreflex following 0.1 µl microinjection within the NTS of either PTK inhibitor genistein(10 mol/L), AMPA glutamate receptor inhibitor CNQX (10 mol/L),or inactive PTK inhibitor daidzein (10 mol/L) were recorded. The results are as follows: Both genistein and CNQX attenuated the ventilatory responses of peripheral chemoreflex, while no changes occurred following daidzein. The amplitude of integrated phrenic nerve discharge and the phrenic burst frequency were decreased by (−21.77±6.93)% and (−24.70± 7.61)% respectively after administration of g