健康食品等に含有するポリフェノール類等の一斉分析によ.PDF

22 13 2010 Toshihiro OHKURA, Minako MOCHIZUKI, Michiyo OHNISHI, Yuzou OKA, Mitsunori DOI An analytical method using liquid chromatography tandem mass spectrometry (LC/MS/MS) and liquid chromatography Time-of-Flight mass spectrometry (LC/TOF-MS) equipped with electrospray ionization (ESI) was demonstrated for the determination of amount of polyphenols, such as anthocyanins (5 compounds), flavanones (5 compounds), flavones (10 compounds), flavonols (6 compounds), isoflavones (6 compounds) and anthraquinones (3 compounds), catechins(4 compounds), and furanocoumarins (4 compounds) in so-called health-promoting food. The HPLC eluted analytes within 32 min, and analytical cycle was 50 min by a sample. The sample was crushed, powdered with mortar, extracted with methanol and subjected to LC/MS/MS and LC/TOF-MS without hydrolysis. The limit of quantification of developed method was 0.01-0.56μg/g(S/N=10) in samples. Amount of individual compound of polyphenols and furanocoumarins (43 compounds) was determined by analyzing each of 39 health-promoting food items which sell in the high-ranking of all obtained in Japan and the daily intake of individual compounds was estimated by the amount of compound and the daily intake of individual item . A Study on the daily intake of polyphenols and furanocoumarins through foods obtained in Ehime Prefecture was carried out by the total diet-market basket method on the basis of food classification and the data of food consumption in the Shikoku region obtained from the Japan Nutrition Survey. In several health-promoting food items, isoflavones were detected and the highest daily intake of isoflav