宏基因组学技术的研究和挑战_周丹燕.pdfVIP

微生物学通报 APR 20, 2011, 38(4): 591−600 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 专论与综述 宏基因组学技术的研究与挑战 1,2 1 1 1* 周丹燕 戴世鲲 王广华 李翔 (1. 510301) (2. 100049) 摘 要: 宏基因组学研究作为研究微生物种群生态分布、群体遗传特征和基因相互作用的新兴学 科领域, 在很大程度上促进了环境微生物资源, 特别是未培养微生物资源的开发利用, 在土壤、海 洋、人体医学、药物等各个领域的应用中取得了突破性的进展, 为发现新的生物活性物质提供了 新的有效途径。就宏基因组学研究进展进行综述, 并重点介绍了宏基因组学研究中的机遇及挑战。 关键词: 宏基因组学, 宏基因组文库, 序列筛选, 功能筛选, 新一代测序 The research innovation and challenges in metagenomics ZHOU Dan-Yan1,2 DAI Shi-Kun1 WANG Guang-Hua1 LI Xiang1* (1. Key Laboratory of Marine Bio-resources Sustainable Utilization, Key Laboratory of Marine Drug, South China Sea Institute of Oceanology Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China) (2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China) Abstract: As the new scientific field of studying the gene function and interactions of microbial com- munity, metagenomics has largely contributed to the development and utilization of microbial re- sources, and made breakthrough progress in various fields, such as soil, oceans, human medical, phar- maceutical and so on. Metagenomics provided a novel approach for the discovery of new bioactive substances. Here we discuss some recent progress with emphasis on the opportunities and challenges in metagenomics application