不同贮藏年限青龙衣中羟基萘醌比较.PDFVIP

现代药物与临床 Drugs Clinic 第 26 卷 第 3 期 2011 年 5 月 • 221 • 不同贮藏年限青龙衣中羟基萘醌的比较 辛国松 1, 2，曲中原 1, 2，邹 翔 1, 2，李 钧 1, 2，季宇彬 1, 2 1. 哈尔滨商业大学 生命科学与环境科学研究中心，黑龙江 哈尔滨 150076 2. 国家教育部抗肿瘤天然药物工程研究中心，黑龙江 哈尔滨 150076 摘 要：目的 建立测定青龙衣中羟基萘醌的方法，考察不同贮藏年限的青龙衣药材中羟基萘醌的变化。方法 以胡桃醌为 对照品，5%氢氧化钾为显色剂，在 515 nm 波长下，采用比色法测定青龙衣中羟基萘醌，对不同贮藏年限的青龙衣药材中羟 基萘醌进行比较。结果 贮藏年限为一年、二年和三年的青龙衣药材中羟基萘醌的量存在明显差异，即随着贮藏时间的延长， 青龙衣中羟基萘醌的量逐渐下降。结论 青龙衣药材不宜储藏时间过长，尽量应用当年的药材。 关键词：青龙衣；羟基萘醌；胡桃醌；贮藏年限；比色法 中图分类号：R282.4 ；R286.0 文献标志码：A 文章编号：1674 - 5515(2011)03 - 0221- 03 Comparison of hydroxyl naphthoquinones from Qinglongyi in different storage times XIN Guo-song1,2, QU Zhong-yuan1,2, ZOU Xiang1,2, LI Jun1,2, JI Yu-bin1,2 1. Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China 2. Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin 150076, China Abstract: Objective To establish a determination method of hydroxyl naphthoquinones for Qinglongyi (fresh rejuvenated fruits of Juglans mandshurica), and to compare hydroxyl naphthoquinones from Qinglongyi in different storage times. Methods Juglone was used as standard, 5% KOH solution was used as chromogenic reagent, and wavelength was at 515 nm. Hydroxyl naphthoquinones in Qinglongyi in different storage times were determined by colorimetric method and compared. Results There were great differences among hydroxyl naphthoquinones contents of storage period 1, 2 and 3 years of Qinglongyi. The hydroxyl naphthoquinones contents of Qinglongyi gradually decreased with the storage time prolonged. Conclusion Qinglongyi should not be kept too long time, and should be used that very year. Key word: Qinglongyi; hydroxy naphthoquinones; juglone; storage time; colorimetry 青龙衣是胡桃科胡桃树属