蒲葵种子脱水耐性及脱水对其膜脂过氧化的影响.pdfVIP

园 艺 学 报 2011，38（8）：1572–1578 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 蒲葵种子脱水耐性及脱水对其膜脂过氧化的影 响 李 朋，唐安军，柳建平，罗安才* （重庆师范大学生命科学学院，重庆 400047 ） 摘 要：研究了脱水对蒲葵种子活力及其膜脂过氧化的影响。结果表明：当蒲葵种子含水量下降到 12%时，发芽率降至 15%，含水量下降到 8%时活力完全丧失，种子对脱水敏感，为顽拗型种子。在脱水 过程中，种子发芽率、发芽势和发芽指数逐渐降低；膜脂过氧化程度加强，相对电导率、丙二醛（MDA ） 和脯氨酸的含量不断上升；超氧化物歧化酶（SOD ）活性先上升，在含水量22% 时出现最大值，之后急速 下降；过氧化氢酶（CAT ）活性随含水量的下降呈上升趋势；过氧化物酶（POD ）和抗坏血酸过氧化物酶 （APX ）变化趋势相同，均随着含水量的降低先上升，在含水量为17%时最高，然后逐渐下降。这些结果 表明，严重脱水能降低蒲葵种子的抗氧化酶活性，并加剧膜脂过氧化，从而导致种子活力降低，甚至丧 失。 关键词：蒲葵；顽拗性种子；脱水耐性；生活力 中图分类号：S 687 文献标识码：A 文章编号：0513-353X （2011 ）08-1572-07 Desiccation Tolerance of Livistona chinensis Seeds and Effect of Desiccation on Peroxidation of Their Membrane Lipid * LI Peng ，TANG An-jun ，LIU Jian-ping ，and LUO An-cai （College of Life Sciences，Chongqing Normal University，Chongqing 400047 ，China ） Abstract ：The paper focused on the effects of desiccation on viability and membrane lipid peroxidation （MLP ）in seeds of Livistona chinensis. Seed germination percentage declined to 15% when moisture content was reduced to 12% ，and then completely lost at 8% of water content，which suggested that Livistona chinensis seeds were desiccation-sensitive seeds，ranked as recalcitrant. In the process of dehydration，the germination percentage ，germination strength and germination index of seeds were gradually decreased. The electrical conductivity，malondialdehyde （MDA ）and proline contents were progressively increased ，which was caused by increased enhanced MLP. Superoxide dismutase （SOD ） activity increased to the maximal value at 22% of seed moisture content，then declined rapidly with further water loss. However ，catalase （C