2014年6月26日 生物信息学相关知识进期学习汇报.pptx

生物信息学相关知识进期学习汇报;在小鼠对乙酰氨基酚肝毒性耐受模型中基因表达的变化分析 2014年发表在《Toxicology and Applied Pharmacology》 《应用毒理学和药理学》杂志 2013年影响因子：3.975;Dosing regimen 1: Following overnight fast, mice were treated with APAP (400 mg/kg) in 50% propylene glycol or vehicle; then, 48 h later, APAP and vehicle pretreated animals were treated with either APAP (600 mg/kg) in 50% propylene glycol or vehicle (5 mL/kg i.p.). Liver and plasma were collected 4 or 24 h later. Dosing regimen 2: Following overnight fast, mice were treated with APAP (400mg/kg) in 50% propylene glycol or vehicle (5mL/kgi.p.). Liver and plasma were collected 2, 4, 8, 12, 24 and 48 h later. Dosing regimen 3: Following overnight fast, mice were treated with APAP (400 mg/kg) in 50% propylene glycol or vehicle (5 mL/kg i.p.). To block compensatory hepatocyte proliferation, 2 mg/kg colchicine or vehicle (saline; 5 mL/kg i.p.) was given 24 and 49 h later. A second dose of APAP (600 mg/kg) or vehicle (50% propylene glycol, 5 mL/kg i.p.) was administered 48 h after the initial APAP dose. Plasma and livers were collected 24 h after the second dose of APAP.;饲养方法三：; (A) VV24 (B) AV24 (C) VA24 (D) AA24;肝小叶中央区着重染色;饲养方法三：;通过生物信息学方法分析卵巢癌发病过程中的关键基因及相关机理 2013年发表《Journal of Ovarian Research》 卵巢研究杂志 2013年影响因子：2.429 作者单位：中国医科大学附属盛京医院妇产科;GSE14407数据集 12个卵巢癌+12个正常芯片;通过细胞色素P450代谢的外源性化学物质;VEGFA,CALM1, IRC5, POLD1, AURKA, CDT1, BUB1B were selected as hub nodes as their connectivity degrees 30.;;有丝分裂细胞循环;卵母细胞减数分裂;CCNE1编码的蛋白是属于高度保守的蛋白家族，他是CDK2的调节亚基，并且他的活性是细胞周期的G1/S过度所必需的。在先前的研究中也表明它的过表达可以提示卵巢癌肿瘤生长和患者的生存状况。;Identification of DEGs After obtaining the raw data, the RMA (Robust Multiarray Average) method [14] of the R software [15] was used to perform quartile data normalization, then the t test methods of the Limma package [16] was used to identify DEGs. Values of |log Fold Change (FC)| 2.0 and p-value 0.05 were selected as the cut-off criteria.;Protein-protein interaction network construction（PPI） Since proteins seldom perform their functions in isolati