纳米炭-表阿霉素混悬液对人乳腺癌mda-mb-231细胞增殖的抑制作用 ....docVIP

纳米炭-表阿霉素混悬液对人乳腺癌MDA-MB-231细胞增殖的抑制作用 张永松，梁全琨，钟 玲，齐晓伟，宗贝歌，杜俊泽，岳志强，张 毅，范林军 （400038 重庆，第三军医大学西南医院乳腺中心） [摘要] 目的 探讨纳米炭-表阿霉素混悬液（carbon nanoparticles-epirubicin suspension，CNP-EPI）对人乳腺癌MDA-MB-231细胞增殖和凋亡的影响。方法 采用不同浓度的CNP-EPI处理MDA-MB-231细胞，CCK-8法测定不同浓度、不同时间对MDA-MB-231细胞增殖的抑制作用；根据测定的IC50选取5 μg/mL CNP-EPI，相应浓度25 μg/mL CNP及仅加培养基作用24 h后，观察细胞形态的变化,流式细胞仪（flow cytometry,FCM）测定细胞凋亡率。结果 在1～200 μg/mL浓度范围内，CNP-EPI对乳腺癌MDA-MB-231细胞增殖的抑制作用逐渐增强，48 h后仍有增加的趋势，但逐渐趋于稳定，且各时间段内对细胞的抑制率与药物浓度呈剂量依赖关系，与正常对照组比较，差异有统计学意义（P＜0.01）MDA-MB-231细胞增殖有明显抑制或杀伤作用，其抑制作用可能是通过其缓慢释放的表阿霉素直接杀伤癌细胞或诱导其凋亡实现。 [关键词] 纳米炭-表阿霉素混悬液； MDA-MB-231细胞；增殖；凋亡 [中图法分类号] [文献标志码] A Inhibition effect of carbon nanoparticles-epirubicin suspension on the proliferation of human breast cancer MDA-MB-231 cells Zhang Yongsong , Liang Quankun , Zhong Ling , Qi Xiaowei , Zong Beige , Du Junze , Yue Zhiqiang , Zhang Yi , Fan Linjun (Center of Breast Disease，Southwest Hospital，Third Military Medical University，Chongqing,400038,China) [Abstract] Obective To explore the effect of carbon nanoparticles-epirubicin suspension on proliferation and apoptosis in human breast cancer MDA-MB-231 cells.Methods MDA-MB-231 cells were cultured with different concentrations of CNP-EPI in vitro. CCK-8 assay was used for determinate inhibition effect of CNP-EPI on the proliferation of MDA-MB-231 cells at different concentration and different time; According to the determination of IC50, 5?g/ml CNP - EPI, 25?g/ml CNP and only add culture medium were selected, and cell morphology and cell apoptosis rates were observed after 24h. Results The inhibition of CNP–EPI on the proliferation of MDA -MB-231 cells was gradually strengthened in a dose-dependent relation when the concentration was from 1?g/ml to 200?g/ml within same time point, the inhibition effect showed increasing trend after 48 hours, but gradually became stabilization. The inhibition effect of the CNP-EPI was stronger significantly in CNP-EPI group than in normal control group at all time point (