响应面试验优化粗糙脉胞菌中番茄红素的提取工艺.pdfVIP

※王艺技术 昌目耐事 2015, Vo1.36, No.18 75 响应面试验优化粗糙脉胞菌中 番茄红素的提取王艺 欧明花l ，陈钢l ，*，耿英龙1 ，刘晓冬l ，苏伟2 ，简素平l ，李陈陈l (1.南昌大学食品科学与技术国家重点实验室，江西南昌 33伽7; 2 江西科技师范大学生命科学学院，江西南昌 330047) 摘 要:应用响应面分析法对粗糙脉f包菌 (Neurospora crassa) 中番茄红素的提取工艺进行优化。粗糙脉泡 菌 (Neurospora crassa) 3.1607为发酵菌种发酵，研磨结合超声破碎法破碎菌体，以溶剂配比、浸提温度、 浸提时间为影响因素，产物高效液相色谱分析得到提取量为响应值。通过响应面分析法得到最佳提取条件 为浸提温度67 .4 8 oC 、浸提时间2.51 h 、溶剂配比(乙酸乙醋-丙酬， V/V) 16:25 ， 此时番茄红素提取量最高 可达7 1. 227 问/g 。按修正后的最佳工艺条件 (67 oC 、 2.5 h 、溶剂配比2: 引进行验证实验，实际提取结果为 7 1. 688μg/g ，这与模型预测值吻合。 关键词:粗糙脉抱菌:番茄红素:提取优化:高效液相色谱:响应面法 Optimization of Extraction of Intracellular Lycopene from Neurospora crassa by Response Surface Design 1 1 1 1 2 l l OU Minghua , CHEN Gang .* , GENG Ying10ng , LIU Xiaodong , SU Wei , JIAN Suping , LI Chenchen (1. State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang 330047 , China; 2. College of Life Science, Jiangxi Science and Technology Normal University , Nanchang 330047 , China) Abstract: Response surface design was employed to optimize the extraction of intracellular lycopene from Neurospora crassa. The cultured cells of 肌 crassa 3.1607 were disrupted ultrasonically by milling for the extraction experiments which were designed to explore the effects of processing variables (extraction solvent, temperaωre and duration) on the extraction yield of lycopene.ηle optimal extraction conditions were determined as 67 .48 oC , 2.51 h and ethyl acetate-acetone (16:25 , V/ηfor tempera阳妃， time and extraction solvent, respectively. Under these conditions , the maximum predicted yield of lycope