pFB-ERV逆病毒载体使用说明.pdf

pFB-ERV 编号 载体名称 北京华越洋VECT55168 pFB-ERV pFB-ERV 逆病毒载体基本信息： 载体名称: pFB-ERV 质粒类型: 逆病毒载体；双顺反子载体 高拷贝/低拷贝: 低拷贝 克隆方法: 限制性内切酶，多克隆位点 启动子: CMV 载体大小: 11067 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: -- 载体抗性: 卡那霉素 筛选标记: Neomycin1.html target=_blank新霉素 （Neomycin） 克隆菌株: DH5α 等 宿主细胞（系）: 常规细胞系（293、CV-1、CHO 等） 备注: -- 稳定性: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 逆转录病毒 pFB-ERV 载体质粒图谱和多克隆位点信息： pFB-ERV 载体简介： pFB-ERV 载体描述 DNA vector-based systems that allow precise control of gene expression in vivo have become invaluable for the study of gene function in a variety of organisms, particularly when applied to the study of developmental and other biological processes for which the timing or dosage of gene expression is critical to gene function. Such systems have also been successfully used to overexpress toxic or disease-causing genes, to induce gene targeting, and to express antisense RNA. Inducible systems are currently being used by pharmaceutical companies to facilitate screening for inhibitors of clinically relevant biological pathways, and potential applications for gene therapy are being explored. The Agilent Complete Control ecdysone-inducible plasmid vectors are based on the insect molting hormone ecdysone, which can stimulate transcriptional activation in mammalian cells harboring the ecdysone receptor protein from Drosophila melanogaster.2 The system has a number of advantages over alternative systems. Firstly, the lipophilic nature and short in vivo half-life of the ecdysone analog ponasterone A (ponA) allows efficient penetrance into all tissu