Nogo-A及Myf-5在急、慢性脊髓压迫减压后的表达及意义.pdf

生有很强的抑制作用，同时脊髓损伤导致的失神经骨骼肌萎缩后 Myf-5 表 达上调，说明 Myf-5 在防治骨骼肌萎缩和肌细胞凋亡中起着重要的保护作 用，研究表明在相同压迫程度下进行减压，慢性脊髓压迫减压后和急性脊 髓压迫减压后与之比较，慢性脊髓压迫减压后更利于神经和肌肉组织的修 复。 关键词：脊髓损伤；神经抑制生长因子Nogo-A ；成肌调节因子Myf-5； 免疫组化 II NOGO-A AND MYF-5 IN THE ACUTE AND CHRONIC SPINAL CORD COMPRESSION PRESSURE EXPRESSION AND SIGHIFICANCE ABSTRACT Objective：Acute and chronic studying rat spinal cord injury at different time points after decompression Nogo-A and Myf-5 expression, this paper discusses the changes Nogo-A, Myf-5 in spinal cord injury after decompression expressive meaning. Methods ：Age 80 SD rats, only 200 - male 250g weight not limited. Respectively established the acute spinal cord injury model and chronic spinal cord damage model. Set up A group of normal control group, and group B JiaShouShu group, the group C, D chronic oppression reduced pressure group of acute oppression, the pressure groups respectively after decompression, 3d in 0d 20d 10d, drawn,. Application immunohistochemical method observation oppression spinal cord tissues Nogo-A, atrophy Myf-5 expression gastrocnemius changes. HE dyed observation after spinal cord injury and muscle atrophy morphological changes in different period. Results ：Normal control group (group A) and JiaShouShu group (group B) compare Nogo-A expression does not change significantly after decompression, chronic oppression group (group C) and acute oppression group after decompression (D group) Nogo-A and III Myf-5 expression changes remarkably, delayed-type greup significance. Immunohistochemical display: Nogo-A group D after rising pressure 0d express 3.34), plus or minus (junior high (91.1 3d expression plus or minus 327