抗菌肽药物工程化技术研究进展.PDFVIP

微生物学通报 Oct. 20, 2014, 41(10): 2128−2133 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140003 专论与综述 抗菌肽药物工程化技术研究进展 汪小杰 毛若雨 王秀敏 滕达 王建华* ( 100081) 摘 要尽管抗生素在畜牧业疾病防治中的主导地位在短期内不会动摇，但病原菌耐药性形成 与快速发展让抗菌肽成为近年新药研发热点之一。作为一种在生物体内广泛存在的天然免疫物 质，抗菌肽具有抗菌、抗病毒、抗肿瘤和免疫调节等功能，由于药效短、对蛋白酶敏感、细胞 毒性高等缺陷而限制其应用。本文综述了抗菌肽基因工程技术、聚乙二醇(PEG)化、靶向性改 造和固定化等工程技术在抗菌肽新药开发中的应用研究进展，以促进抗菌肽产业化。 关键词抗菌肽，基因工程，聚乙二醇化，靶向性，固定化 Advances on antimicrobial peptide engineering * WANG Xiao-Jie MAO Ruo-Yu WANG Xiu-Min TENG Da WANG Jian-Hua (Key Laboratory of Feed Biotechnology of MOA , Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) Abstract: Although antibiotics hold a leading position in the medical treatment and animal husbandry, but its prone-resistance to the pathogen lead to the rise of research and development of antimicrobial peptides (AMPs). As natural active substances, AMPs have antibacterial, antiviral, anti-tumor and immune regulation function, while the shortcomings such as short efficacy, sensitive to proteinase and high cytotoxicity limit its application. In this paper, the bottlenecks and their solution of antimicrobial peptide engineering were reviewed in terms of gene engineering, PEGylation, targeting and immobilization in order to promote the industrialization of AMPs. Keywords: Antimicrobial peptides, Gene engineering, PEGylation, Targeting, Immobilization (Antimicrobial peptidesAMPs) PR39 Indolicidin