布鲁氏菌野毒株A544与疫苗株104M的差异蛋白筛选.PDFVIP

2017年第34卷第 5 期 布鲁氏菌野毒株A544 与 疫苗株 104M 的差异蛋白筛选 王海艳，赵治国，赵林立，刘　佳，任彩霞，敖威华，马彩霞，李　刚，郭文秀，陈宇飞 （内蒙古出入境检验检疫局，内蒙古呼和浩特　010020 ） 摘　要 ：为给布鲁氏菌病自然感染与疫苗免疫鉴别方法的建立提供参考，采用比较蛋白质组学研究技术，以野 毒株A544 和疫苗株104M 的菌体蛋白为研究对象，以双向电泳技术为分离手段，以基质辅助激光解吸飞行时 间质谱为检测手段，利用生物学信息解析所得实验结果。双向电泳结果分析显示，野毒株A544 和疫苗株104M 之间约有20 个差异蛋白点，经质谱成功鉴定了9 种蛋白。通过蛋白数据库检索发现，这些蛋白主要参与了一些 代谢调节等生物过程，其中有6 个蛋白点在野毒株A544 上高表达，3 个蛋白点在疫苗株104M 上高表达。 关键词：布鲁氏菌野毒株A544 ；布鲁氏菌疫苗株104M ；差异蛋白筛选 中图分类号：S852.6　　文献标识码：B　　文章编号：1005-944X （2017 ）05-0101-05 DOI ：10.3969/j.issn.1005-944X.2017.05.028 Screening of Differential Proteins between Brucella Wild Strain A544 and Vaccine Strain 104M Wang Haiyan ，Zhao Zhiguo ，Zhao Linli ，Liu Jia ，Ren Caixia ，Ao Weihua ， Ma Caixia ，Li Gang ，Guo Wenxiu，Chen Yufei （Inner Mongolia Entry-exit Inspection and Quarantine Bureau，Huhhot ，Inner Mongolia　010020 ） Abstract ：Objective to provide reference for developing a method to distinguish wild-type strains of Brucella from vaccine strains ，differential proteins in wild strain A544 and vaccine strain 104M were screened and identified by comparative proteomics approach. Firstly ，using two-dimensional gel electrophoresis to separate the differential proteins ，then detecting these proteins by matrix-assisted laser desorption-ionization time of flight mass spectrometry （MALDI-TOF MS ），at last ，analyzing related biological information to get the results. Based on the analysis of two-dimensional gel electrophoresis ，about 20 differential proteins were separated，and 9 of them were successfully identified through the mass spectrometry. NCBI protein database searching demonstrated that these proteins were mainly involved in metabolic regulation in some biological process. Among them，6 proteins showed