草履虫(国外英语资料).docVIP

草履虫 Paramecium culture (backwaters) Paramecium is an important representative of protozoa, is also a beginner learn the first contact with the animal animal. As the representative of the ciliate Paramecium to have a very important value in water purification and monitoring. This experiment is mainly introduced and various method of cultivation and purification of Paramecium, and through observation, grasp the basic characteristics of protozoa, and better understand the knowledge about other protozoa. Experimental preparation (I) materials Dry straw, old straw mat, dry corn tassel, leaf and stem of wheat grain, from guava, dry yeast, animal offal (liver, pancreas, thyroid), egg yolk, beef juice (50g beef, 100 g water, cooked). (two) supplies Microscope, magnifying glass, electric furnace, incubator, slide cover glass, dropper, micro Straw, dissecting needle, beaker, Petri dishes, concave glass, gauze, absorbent paper, pins, agar, mass fraction of methyl cellulose solution, 3% iodine solution, glucose, sodium chloride, the mass fraction of magenta the solution, 1% of the 0.1% mass fraction of neutral red solution. (three) collection and culture 1. acquisition Select multiple sites for collection. Any organic content of sewage can be collected 250~500 mL water samples, on the spot with a magnifying glass examination. Sample examination, who was white, larger, terete, slightly rounded end end slightly sharp animal is paramecium. If multiple checks are not found Paramecium, water can be discarded, choose other acquisition. Water samples were retrieved by microscopic examination, all containing Paramecium in water samples from water bottles at 10 ~ 20cm, placed by a lamp, 24h lighting, the Paramecium gathered to the surface of the water. 2. culture medium preparation There are many preparation methods, which can be chosen according to the teaching practice. (1) straw cultivation liquid dried straw 10g, cut into 3Cm long, put people beaker, add water 1000 M1, boil 10~15 m