硫酸盐还原菌的培养驯化及其脱硫性能.pdfVIP

中连续的线性关系较好的点拟合得的一次函数的斜率即为细菌的比生 长速率 μ ，该值的大小直接反应了菌种在对数生长期的生长快慢情况， 从而反应了硫酸盐还原菌的生长规律。 得出在最优培养条件下 μ 的值为 0.02531h-1 。 关键词：微生物代谢；硫酸盐还原菌；分离纯化；脱硫性能；比生长速率 Abstract In this thesis, sulfate-reducing bacteria (SRB-DYB2) was selected for studying，which could deoxidize S6+ to S or S2-, on account of thermal power plant desulfurization of fluid which could cause pollution for the environment，the main purpose is to train with the desulfurization performance DYB2 bacteria.The colony was isolated from Hohhot chemical soil with sulfate, Baotou mine water and soil samples, and Wudalianchi water samples, using dilution spread and repeat dish sandwish cultivated method, plate pyrogallic acid method and three roller tube culture methods, DYB2 were screened and enriched, to be a single pure colony. It was identified that the Young DYB2 cells were elongated, irregularly globular, 0.4 ~ 0.8μm × 1.0 ~ 4.0μm, single or in pairs, not a common branch, not the formation of mycelium, single polar flagella. The bacteria colony to oval, size of 2 ~ 3mm, moist surface opaque, , thick, irregular edge of the central uplift, villous surface, the cells stain Gram-negative.The DYB2 strain belongs to typical Desulfosarcina. The experiment on sulfate-reducing bacteria’s growth conditions was investigated. Respective effects of temperature, pH, shaking speed, S2-concentration and Fe2+ concentration on the growth of SRB, the optimization growth conditions was measured.With the optimum conditions, the growth of SRB in accordance with the fixed time made the growth of curve.Anaerobic bacteria sulfate-reducing bacteria (SRB)’s best