人结肠癌组织中Raf-1的表达及其临床意义.pdfVIP

中国肿瘤生物治疗杂志 Vo1．14 No．6 Dec．20o7 Chinese Journal of Cancer Biotherapy ·571· [文章编号] 1007-385X(2007)06-0571-04 · 论 著· 人结肠癌组织中Raf-1的表达及其临床意义 张宁宁，刘文超 ，薛 研(第四军医大学西京医院肿瘤科，西安 710032) [摘 要] 目的：研究Raf-1在人结肠癌组织中的表达与肿瘤血管生成的关系，并探讨它们的临床意义。方法：应用组织芯 片技术和免疫组化法检测来自西京医院病理科2005—2006年间的87例结肠癌病例癌区、癌旁区及切缘区组织标本中Raf-1的 表达，应用CD34标记的免疫组化法检测微血管密度(microvessel density，MVD)；并分析Raf-1表达和MVD与结肠癌体积、转移、 分化等病理特征之间的相关陛。结果：Raf-1在癌组织、癌旁组织、切缘区组织的阳性率分别为86．47％、37．34％和11．03％，3 者比较有显著性差异(P0．01)。癌组织、癌旁组织、切缘区组织中MVD值分别为(61．35±11．6O)、(28．34±6．70)和(8．26± 3．70)，3者有显著性差异(P0．O1)。Raf-1表达和MVD与肿瘤大小、转移与否密切相关。结论：Raf-1是调控人结肠癌组织血 管生成的关键分子，在癌组织中Raf-1的表达与MVD值成正相关，两者与临床上肿瘤的大小、转移与否密切相关。 [关键词] Raf-1，肿瘤血管生成，组织芯片，微血管密度，结肠癌 [中图分类号] R735．3；R730．2 [文献标志码] A Expression of Raf-1 in colon carcinoma tissue and its clinical significance ZHANG Ning-ning，LIU Wen-chao ，XUE Yan(Department of Clinical Oncology，Xijing Hospital，Fourth Military Med- ical University，Xi an 710032，China) [Abstract] Objective：To investigate the relationship between the expression of Raf-1 and tumor angiogenesis，and to discuss its clinical significance．Methods：Tissue microarray technique was used to detect the expression of Raf-1 in 87 specimens of human colon carcinoma，their corresponding adjacent tissues，and incision margins．The patients were from the Department of Pathology of Xijing Hospital between 2005 and 2006．Microvessel density(MVD)was detected using immunohistochemistry with CD34 labeling．The correlation between Raf-1 expression and MVD with the tumor size，metas‘ tasis，and differentiation was analyzed．Results：The positive rates of Raf-1 in colon carcinoma tissues，adjacent tissues and incision margins were 86．47％，37．34％ and 11．03％，respectively；there were significant difference among the 3 values(P0．01)．The MVD in 3 regions were(61．35±11．6)，(28．34±6．7)，and(8．26±3．7)，respectively； and there