重组PP150抗原用于胶体金免疫斑点技术检测抗HCMV_IgM.pdfVIP

1764 ( J Fourth M ilM ed Univ) 2005; 26( 19) http: / /journal. fmm u. edu. cn : 1000-2790( 2005) 19-1764-04 PP150 HCMV-IgM 1 1 1 1 2 2 王 清 , 王锡波, 徐龙强, 于维林 , 于 红, 张文卿 1 2 ( : , , 266003) R ecom binant PP150 of hum an cyto- ELISA , 96. 0% ; CGIDA HCMV-IgM 74. 2% , 100% . : pp150 m egalovirus as antigene for colloidal CG IDA HCMV, . gold imm uno-dot assay to detect HC- 巨细胞病毒, 人; 基因 pp150; 原核表达; 胶体金; 免 MV-IgM in sera 疫 点法 1 1 1 1 R373 A WANG Q ing , WAN G X i-B o , X U L ong-Q iang , YU W ei-L ing , 2 2 YU H ong , ZHANG W en-Q ing 1 Departm ent of C lin ical Laboratory, A ffiliated H ospital, 0 2 Departm ent of M ed icl M icrobiology, M ed ical College, Q ingdao ( colloidal gold mi muno-dot as- Un iversity, Q ingdao 266003, Ch ina [ 1] say, CG IDA ) , CGIDA HCMV-IgG , AbstractA IM To use recombinant pp150 of human cyto- ( HCMV )-IgM . megalovirus ( HCMV ) in colloida l godl mi muno-dot assay HCMV pp150( 841~ 1084aa) ( CG ID ) for the detection of HCMV-IgM in sera. M ETH- , , OD S fragment ofgene coding for pp150 antigen epitopes pp150 IgM . ( 840 - 1048aa) was expressed inE. co li and the fusion proteinwas purifei d. The resultswere compared w ith those of specifci antibodei s ELIS IgM. RE SULT S Recombinant 1 antgi en CG ID had a good agreement ( 96. 0% ) w ith 1. 1 HCMV AD169 E. coli ELIS ki.t The spec if