中分子量羟乙基淀粉对脊髓损伤后神经病理性疼痛 - 第三军医大学学报.DOC

中分子量羟乙基淀粉对脊髓损伤后神经病理性疼痛的影响 胡新宇，陶 军，杨天德，谢科宇 (400037 重庆，第三军医大学新桥医院麻醉科) [摘要] 目的 研究对脊髓损伤后的影响。随机低分子量）））hydroxyethyl starch on neuropathic pain following spinal cord injury Hu Xinyu，（nesthesiology，Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China) [Abstract] Objective To investigate the effects of medium molecular weight hydroxyethyl starch (HES 130/0.4, Voluven) on below-level neuropathic pain following spinal cord injury (SCI). Methods Using random number method, totally 72 SD adult male rats were randomly and equally divided into 4groups: moderate spinal cord contusion (T10-11) and saline i.v., SCI and HES 40 i.v., SCI and HES 130/0.4, laminectomy control. In ethology, paw withdraw pressure threshold (PWPT) were observed to evaluate the degree of central pain. We measured the evans blue (EB) loading uptake in spinal cord injury to evaluate the disruption of blood spinal cord barrier.Western blotting for GFAP, Iba1 and ED1 were used to observe the astrocytes and macrophage/microglia activation, and Elisa to quantitate the expression of IL-1, IL-6, TNF-α. Results HES130/0.4 retrieved the compromised BSCB after 3 days of spinal cord injury comper with normal saline and low molecular weight hydroxyethyl starch（Saline: 2.58±0.30; HES 40: 2.38±0.48; HES 130/0.4: 1.38±0.35））immunohistochemistry test were performed to observe the histomorphology lessened the macrophage/microglia activation and reduced the expression of IL-1, IL-6, TNF-αin injured segments（P0.05） normal saline and low molecular weight hydroxyethyl starch（P0.05）Conclusion An administration of HES 130/0.4 could attenuated the below-level neuropathic pain following spinal cord injury, the underlying mechanism may be to remedy the BSCB disruption, inhibit the macrophage/microglia activation . [Key words] spinal cord injury; neuropathic pain; blood spinal cord barrier; hydroxyethyl starch; inflammation Corresponding author:Tao Jun, E-mail: xqtj0560@