不同结构生物组织光透明作用比较.pdfVIP

中国激光 Vo l. 33 , No.7 第 33 卷第7 期 2006 年 7 月 CHINESE JOURNAL OF LASERS July , 2006 文章编号: 0258-7025(2006)07-0998-05 不同结构生物组织光透明作用比较 徐向群1 ，关抑2 摘要 为了了解生物组织不同结构对光透明进程的影响，利用光相干断层成像术(OCT) 和近红外光谱(NIR) 对肌 肉组织(猪骨锵肌)和上皮组织(猪因蒙古膜)经丙三醇处理后的光透明效果进行了比较研究。结果表明，这两种组织 的光相干断层成像术成像深度和内层次结构的成像对比度均得到改善;肌肉组织和罔秸膜组织的光透射率分别增 加了 21%和 16% ，而漫反射率分别降低了 33%和 21% ;这两种结构组织的光透明效果(肌肉组织较好)和进程(肌 肉组织较快)与各自的失水率和失水动力学成正相关，肌肉组织失水过程为先快脂慢;同黠膜组织的失水过程为先 慢后快;肌肉组织的总失水率大于商蒙古膜组织。这表明结构不阔的生物组织的光透明效果存在差异，必须考虑其 内部结构的影响。 关键词 医用光学和生物技术;:1:物组织光透明;光相干断层成像术;近红外光谱;:1:物组织结构 中团分类号 R318.51 文献标识码 A Dependence of Optical Clearing Effect on Tissue Structure XU Xiang-qun 1 , WU Liu2 (川川川e ， 川ωω叫ω1愣H 川川川[];川h 2Deρartme叫t 01 Physics , Beijing Jiaotong University. Beijing 100044 , China Abstract The dependence of optical clearing on properties of the different tissue structures is investigated in order to better know the clearing mechanisms and effective clearing approach. Two typical tissues , muscle and epithelial. have been chosen as the targets and the effect of optical clearing has been comparatively studied by optical coherence tomography (OCT) and near infrared spectroscopy (NIR) with the clearing agent glycerol used. 丁he results show that the improved imaging depth and contrast among different layers in the porcine skeletal muscle and stomach mucosa are visualised with the OCl、 assessment. Th日 overall increases in light transmittance in the mu时le tissue and the stomach mucosal tissue after 30 min treatment are observed to be approximate 21