大孔吸附树脂纯化唐古特白刺红色素的研究.pdfVIP

天然产物研究与开发 Nat Prod Res Dev，2007，19：102—106 文章编号：1001-6880(2007)Suppl-0102-05 大孑L吸附树脂纯化唐古特白刺红色素的研究 王凌云 ，丁晨旭 ，索有瑞h ‘中国科学院西北高原生物研究所，西宁810001； 中国科学院研究生院，北京100049 摘 要：通过静态吸附解析实验，确定了利用大孔吸附树脂初步纯化唐古特白刺红色素的条件，并根据所得条 件做了扩大实验。结果表明：X-5树脂的吸附能力最好，吸附率为76．85％，pH在1—3范围内对吸附效果无影 响；较高的乙醇浓度和pH值有利于色素的解析，解析液以80％酸化乙醇(含l％HC1)效果最佳，解析时间为 40 min；扩大实验得到紫红色色素粉末，得率为1．24％，水溶性好，不含糖类、蛋白质等水溶性杂质；树脂通过回 收再生可重复利用。本实验为色素产品的开发及白刺果实的综合利用提供了理论依据。 关键词：唐古特白刺；色素；大孔树脂；吸附；纯化 中图分类号：Q503 文献标识码：A Study on Purification Red Pigment from Nitraria tangutorum Bobr．with Macroporous Resin WANG Ling—yun ’ ，DING Chen—XU ’ ，SUO You—rui ‘Northwest Institute ofPlateau Biology，Chinese Academy ofSciences，Xining 810001，China； Graduate School ofthe Chinese Academy ofSciences，Beijing 100049，China Abstract：The purification conditions of Red pigment from Nitraria tangutorum Bobr．using macroporous resin were con— firmed by adsorbing and desorption experiment and enlarged experiment was studied．The results showed that X-5 resin had more strong adsorption capacity，the adsorptive rate of pigm ent reached 76．85％ ，and pH 1—3 had no influence on adsorbing effect；higher conceutration of ethanol and acidity had a higher desorption ability for red pigment，80％ethanol containing 1％ Hydrochloric acid had the best desorption effect，and the time of desorption were 40 min；we got powder of red pigment through enlarged experiment and the rate of pigment Was 1．24％．This powder had good water-solubi~ty and did not contain some impurities such as glucide，protein．After experiment，the resin could be recycled。These resear- ches prodded academic basis for the exploitation of pigment productions from Nitraria tangutorum Bobr．and comprehen— sive use ofits fruit． Key