毛细管电泳药物分析原理及应用.ppt

200807Chen 毛細管電泳藥物分析原理及應用 This has traditionally been carried out using gas chromatography (GC) and high-performance liquid chromatography (HPLC). Capillary electrophoresis has been investigated as an alternative separation technique for forensic analysis. It has the potential to provide far more rapid separations than are generally achievable with HPLC, and can provide a separation where the analyte in question behaves poorly under GC analysis. Capillary zone electrophoresis (CZE), in which ionic species are separated according to their mobility and polarity in aqueous solution, is the most frequently used option in CE. The term ‘capillary electrophoresis’ describes a family of related techniques in which separations are carried out in arrow bore capillaries under the influence of an electric field. The separations obtained by capillary electrophoresis are highly efficient, rapid, and may be applied to both charged and neutral species. Anon-line search of the Scifinder Scholar database revealed in excess of 30,000 eferences to capillary electrophoresis to date with applications in a wide range ofareas, including pharmaceuticals, food and beverages, environmental and clinical analysis. The potential of this technique for forensic analysis was first demonstrated in 1991 by Weinberger and Lurie, who applied it to the analysis of a wide range of illicit drugs in synthetic mixtures. The exceptional power of separation and resolution, rapid analysis time, low mass detection limits, economy of reagents, and minimum sample requirements make capillary electrophoresis an attractive methodology for forensic laboratories. Capillary electrophoresis Electrophoresis can been defined as the differential migration of charged species (ions) in an electric field, and was first described as a separation technique by Tiselius in 1937. His work, involving the separation of proteins, placed between buffer solutions in a tube across which an electric field was applied, earned him the No